Brief Article
Copyright ©2011 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Feb 14, 2011; 17(6): 789-795
Published online Feb 14, 2011. doi: 10.3748/wjg.v17.i6.789
An end-to-end anastomosis model of guinea pig bile duct: A 6-mo observation
Xiao-Qing Zhang, Yuan-Hu Tian, Zhi Xu, Li-Xin Wang, Chun-Sheng Hou, Xiao-Feng Ling, Xiao-Si Zhou
Xiao-Qing Zhang, Yuan-Hu Tian, Zhi Xu, Li-Xin Wang, Chun-Sheng Hou, Xiao-Feng Ling, Xiao-Si Zhou, Department of General Surgery, Peking University Third Hospital, Beijing 100191, China
Author contributions: Zhang XQ and Tian YH contributed equally to this work, and performed the majority of experiments; Xu Z, Wang LX and Hou CS designed the experiment and provided the professional consults to this research; Zhang XQ wrote the manuscript; Xu Z, Ling XF and Zhou XS revised the manuscript.
Supported by A grant from National 863 Program of China, No. 2007AA04Z313
Correspondence to: Zhi Xu, Professor, Department of General Surgery, Peking University Third Hospital, 49 North Garden Rd, Haidian District, Beijing 100191, China. xuzhi123456@sohu.com
Telephone: +86-10-82267331 Fax: +86-10-62010334
Received: October 17, 2010
Revised: November 14, 2010
Accepted: November 21, 2010
Published online: February 14, 2011
Abstract

AIM: To establish the end-to-end anastomosis (EEA) model of guinea pig bile duct and evaluate the healing process of bile duct.

METHODS: Thirty-two male guinea pigs were randomly divided into control group, 2-, 3-, and 6-mo groups after establishment of EEA model. Histological, immunohistochemical and serologic tests as well as measurement of bile contents were performed. The bile duct diameter and the diameter ratio (DR) were measured to assess the formation of relative stricture.

RESULTS: Acute and chronic inflammatory reactions occurred throughout the healing process of bile duct. Serology test and bile content measurement showed no formation of persistent stricture in 6-mo group. The DR revealed a transient formation of relative stricture in 2-mo group in comparation to control group (2.94 ± 0.17 vs 1.89 ± 0.27, P = 0.004). However, this relative stricture was released in 6-mo group (2.14 ± 0.18, P = 0.440).

CONCLUSION: A simple and reliable EEA model of guinea pig bile duct can be established with a good reproducibility and a satisfactory survival rate.

Keywords: Animal model; Guinea pig; Anastomosis; Common bile duct; Wound healing