Original Article
Copyright ©2011 Baishideng Publishing Group Co.
World J Gastroenterol. Jul 21, 2011; 17(27): 3220-3228
Published online Jul 21, 2011. doi: 10.3748/wjg.v17.i27.3220
Table 1 Primer sequences for quantitative real-time polymerase chain reaction
GenesPrimer sequences (5'-3')ChromosomePCR products (bp)
LYVE-1F: CCAGTGAGCCGACAGTTTGCAG
R: CAGGTATTGTAGAGTAAGGGGATGCC11p15184
VEGFR-3F: ACGGCCTGGTGAGTGGC
R: CGTTTGACTCCTCCGTGATG5q35.363
CD44F: GCAACTCCTAGTAGTACAACGGAAGA
R: CGATATCCCTCATGCCATCTGA11p1380
GAPDHF: GGCTGAGAACGGGAAGCTTGTCAT
R: CAGCCTTCTCCATGGTGGTGAAGA12p13143
Table 2 Clinicopathological features and expression levels of lymphatic vessel endothelial hyaluronan receptor-1, vascular endothelial growth factor receptor-3 and CD44 genes in patients with gastric cancer
FeaturesnLYVE-1PCD44PVEGFR-3P
Lauren type
Intestinal150.76 (0.04-2.66)NS1.14 (0.17-2.80)NS1.20 (0.02-3.70)NS
Diffuse180.81 (0.09-3.12)1.14 (0.28-2.51)0.87 (0.26-1.83)
Differentiation
Well140.80 (0.04-2.66)NS1.14 (0.17-2.80)NS1.23 (0.02-3.70)NS
Poor190.78 (0.09-3.12)1.14 (0.28-2.51)0.86 (0.26-1.83)
T-stage
T220.54 (0.15-0.93)NS0.39 (0.17-0.60)NS0.6NS
T3170.69 (0.04-2.66)1.17 (0.28-2.80)0.99 (0.02-2.94)
T4141.0 (0.10-3.12)1.24 (0.47-2.14)1.0 (0.26-3.70)
Vascular invasion
Negative150.82 (0.15-1.68)NS1.80 (0.60-2.80)NS1.06 (0.60-1.57)NS
Positive180.96 (0.10-3.12)1.07 (0.28-2.14)1.13 (0.26-3.70)
Perineural invasion
Negative100.32 (0.10-0.93)0.0200.57 (0.17-0.76)0.0200.8 (0.26-2.21)NS
Positive231.0 (0.04-3.12)1.40 (0.31-2.80)1.13 (0.02-3.70)
Neural invasion
Negative150.78 (0.04-3.12)NS0.95 (0.17-2.80)NS0.93 (0.02-2.94)NS
Positive180.84 (0.21-2.22)1.48 (0.46-2.51)1.24 (0.40-3.70)
Lymph nodes
Negative50.66 (0.15-1.68)NS0.74 (0.17-1.61)NS0.86 (0.30-2.21)NS
Positive280.82 (0.04-3.12)1.22 (0.28-2.80)1.06 (0.02-3.70)
PLN/TLN ratio
≤ 0.4250.53 (0.04-1.68)NS1.06 (0.17-2.80)NS0.83 (0.02-2.21)NS
> 0.482.0 (0.69-3.12)0.0031.53 (0.47-2.40)NS1.97 (0.28-3.70)0.050