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©2011 Baishideng Publishing Group Co.
World J Gastroenterol. Apr 14, 2011; 17(14): 1903-1909
Published online Apr 14, 2011. doi: 10.3748/wjg.v17.i14.1903
Published online Apr 14, 2011. doi: 10.3748/wjg.v17.i14.1903
Table 1 Forward and reverse primers used in quantitative real-time polymerase chain reaction
Gene | Primer sequence (5’-3’) | Annealing temperature (°C) | Product size (bp) |
β-actin | 5’-ACTGCCGCATCCTCTTCCTC-3’ 5’-ACTCCTGCTTGCTGATCCACAT-3’ | 55 | 598 |
Rat TNF-α | 5’-GGCAGGTCTACTTTGGAGTCATTGC-3’ | 58 | 318 |
5’-ACATTCGGGGATCCAGTGAGCTCCG-3’ | |||
Rat IL-6 | 5’-GGATACCACCCACAACAG-3’ 5’-GGTCCTTAGCCACTCCTT-3’ | 58 | 451 |
Table 2 Mortality of rats in different groups
Table 3 Distribution of endotoxin levels in serum of different groups
- Citation: Yang DH, Ye ZY, Jin B, He XJ, Zhang Q, Zhou WM, Xu WJ, Lu HX. Salvianolate inhibits cytokine gene expression in small intestine of cirrhotic rats. World J Gastroenterol 2011; 17(14): 1903-1909
- URL: https://www.wjgnet.com/1007-9327/full/v17/i14/1903.htm
- DOI: https://dx.doi.org/10.3748/wjg.v17.i14.1903