Omeprazole decreases magnesium transport across Caco-2 monolayers

Table 1 Kinetic data of Mg_B→A transport

	Vm (nmol/hper cm2)	Km (mmol/L)	m (× 10-3 cm/h)
14 d
Control	57.22 ± 8.41	5.62 ± 1.83	2.18 ± 0.11
Omeprazole treated (ng/mL)
200	58.12 ± 6.19	4.55 ± 1.23	1.80 ± 0.08b
400	62.82 ± 9.64	5.83 ± 2.22	1.54 ± 0.12b
600	57.01 ± 7.49	4.48 ± 1.44	1.28 ± 0.09b
800	59.35 ± 7.40	4.71 ± 1.40	0.83 ± 0.09b
1000	58.84 ± 7.52	5.84 ± 1.59	0.52 ± 0.10b
21 d
Control	55.82 ± 8.02	5.09 ± 1.89	2.17 ± 0.10
Omeprazole treated (ng/mL)
200	57.81 ± 10.41	7.48 ± 2.24	1.26 ± 0.12bd
400	53.47 ± 7.59	5.10 ± 1.87	1.18 ± 0.10bd
600	53.12 ± 7.53	4.42 ± 1.68	0.96 ± 0.09bd
800	57.65 ± 6.76	5.97 ± 1.51	0.53 ± 0.08bd
1000	57.99 ± 7.72	6.09 ± 1.48	0.49 ± 0.07b

^bP < 0.001 vs the age-matched control group,

^dP < 0.001 vs the concentration-matched 14 d-omeprazole-treated groups.

Table 2 Electrical parameters of Caco-2 monolayers

	n	PD (mV)	ISC (mA/cm2)	TER (Ω.cm2)
14 d
Control	9	0.99 ± 0.12	3.09 ± 0.36	322.19 ± 6.37
Omeprazole treated (ng/mL)
200	9	1.03 ± 0.54	2.52 ± 0.39	413.64 ± 12.95
400	9	0.98 ± 0.14	2.24 ± 0.29	433.23 ± 17.66
600	9	1.08 ± 0.15	2.29 ± 0.31	470.35 ± 23.87d
800	9	1.09 ± 0.18	2.30 ± 0.41	483.22 ± 20.20d
1000	9	1.20 ± 0.11	2.41 ± 0.20	502.88 ± 30.99d
21 d
Control	9	1.00 ± 0.15	3.18 ± 0.47	314.05 ± 4.64
Omeprazole treated (ng/mL)
200	9	1.26 ± 0.20	2.48 ± 0.32	485.09 ± 24.36b
400	9	1.13 ± 0.19	2.20 ± 0.32	502.19 ± 27.47d
600	9	1.06 ± 0.13	2.21 ± 0.34	500.33 ± 32.97d
800	9	0.99 ± 0.19	1.97 ± 0.31	481.64 ± 25.48d
1000	9	1.07 ± 0.18	2.06 ± 0.30	500.84 ± 26.61d

^bP < 0.01,

^dP < 0.001 vs the age-matched control group.