Omeprazole decreases magnesium transport across Caco-2 monolayers

doi:10.3748/wjg.v17.i12.1574

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Mar 28, 2011 (publication date) through May 6, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Mar 28, 2011; 17(12): 1574-1583
Published online Mar 28, 2011. doi: 10.3748/wjg.v17.i12.1574

Omeprazole decreases magnesium transport across Caco-2 monolayers

Narongrit Thongon, Nateetip Krishnamra

Narongrit Thongon, Faculty of Allied Health Sciences, Burapha University, Chonburi 20131, Thailand

Nateetip Krishnamra, Consortium for Calcium and Bone Research (COCAB) and Department of Physiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand

Author contributions: Thongon N designed and performed the experiment, analyzed and interpreted the results, wrote and edited the manuscript; Krishmanra N provided the critical experimental tools and edited the manuscript.

Supported by The Thailand Research Fund (to Thongon N), No. MRG5380003

Correspondence to: Narongrit Thongon, PhD, Faculty of Allied Health Sciences, Burapha University, 169 Long-Hard Bangsaen Rd., Saensook, Muang, Chonburi 20131, Thailand. narongritt@buu.ac.th

Telephone: +66-38-393497 Fax: +66-38-393497

Received: June 3, 2010
Revised: July 12, 2010
Accepted: July 19, 2010
Published online: March 28, 2011

Abstract

AIM: To elucidate the effect and underlying mechanisms of omeprazole action on Mg²⁺ transport across the intestinal epithelium.

METHODS: Caco-2 monolayers were cultured in various dose omeprazole-containing media for 14 or 21 d before being inserted into a modified Ussing chamber apparatus to investigate the bi-directional Mg²⁺ transport and electrical parameters. Paracellular permeability of the monolayer was also observed by the dilution potential technique and a cation permeability study. An Arrhenius plot was performed to elucidate the activation energy of passive Mg²⁺ transport across the Caco-2 monolayers.

RESULTS: Both apical to basolateral and basolateral to apical passive Mg²⁺ fluxes of omeprazole-treated epithelium were decreased in a dose- and time-dependent manner. Omeprazole also decreased the paracellular cation selectivity and changed the paracellular selective permeability profile of Caco-2 epithelium to Li⁺, Na⁺, K⁺, Rb⁺, and Cs⁺ from series VII to series VI of the Eisenman sequence. The Arrhenius plot revealed the higher activation energy for passive Mg²⁺ transport in omeprazole-treated epithelium than that of control epithelium, indicating that omeprazole affected the paracellular channel of Caco-2 epithelium in such a way that Mg²⁺ movement was impeded.

CONCLUSION: Omeprazole decreased paracellular cation permeability and increased the activation energy for passive Mg²⁺ transport of Caco-2 monolayers that led to the suppression of passive Mg²⁺ absorption.

Keywords: Magnesium, Paracellular, Proton pump inhibitor, Transepithelial, Tight junction