Proteome of human colon cancer stem cells: A comparative analysis

Table 1 Sequences of primers for RT-PCR and length of RT-PCR products

Genes	Primer sequences	Products
GAPDH	5’-TTGGTATCGTGGAAGGACTCA-3’	270 bp
	5’-TGTCATCATATTTGGCAGGTT-3’
CD133	5’-TGGGGCTGCTGTTTATTATTCT-3’	194 bp
	5’-TGCCACAAAACCATAGAAGATG-3’
CD29	5’-GGAAAACGGCAAATTGTCAG-3’	600 bp
	5’-TTGGGGTTGCACTCACACAC-3’
Mus-1	5’-GGCTTCGTCACTTACATGGACCAGGCG-3’	542 bp
	5’-GGAAACTGGTAGGTGTAG-3’
ABCG2	5’-GGGTTCTCTTCTTCCTGACGACC-3’	398 bp
	5’-TGGTTGTGAGATTGACCAACAGACC-3’
TERT	5’-CGGAAGAGTGTCTGGAGCAA-3’	145 bp
	5-GGATGAAGCGGAGTCTGGA-3
Oct-4	5’-GACAACAATGAGAACCTTCAGA-3’	218 bp
	5’-CTGGCGCCGGTTACAGAACCA-3’
Sca-1	5’-AACCATATTTGCCTTCCCGTCT-3’	135 bp
	5’-CCAGGTGCTGCCTCCAGTG-3’

GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; Mus-1: Musashi-1.

Table 2 Chemosensitivity of SW1116csc and SW1116 cells to chemotherapeutic drugs (mean ± SD)

	IC50 (mg/L)
Drugs	SW1116csc	SW1116 cells	Times (fold)
Paclitaxel	23.4 ± 1.8	1.5 ± 0.2	15.6
Adriamycin	29.7 ± 2.1	4.4 ± 0.4	6.8
Etoposide	9.8 ± 0.3	2.1 ± 0.2	4.7
Cytarabine	34.2 ± 2.5	9.6 ± 0.7	3.6
Fluorouracil	57.7 ± 3.8	26.5 ±1.5	2.2
Cisplatin	11.6 ± 0.9	7.3 ± 0.3	1.6
Mitomycin	0.67 ± 0.04	0.46 ± 0.03	1.5

IC₅₀: The half maximal inhibitory concentration.

Table 3 Differently expressed proteins in SW1116csc and SW1116 cells

Protein	Protein description index	NCBI ID	Theoretical Mr	Theoretical pI	Protein coverage(%)	Summary score
7387	stratifin	gi | 5454052	27774	4.68	3	41
6402	Raichu404X	gi | 14595132	85016	6.45	10	102
6963	Ubiquitin fusion-degradation 1 like protein	gi | 1654346	39170	6.04	7	58
7013	eukaryotic translation elongation factor 1 delta isoform 2	gi | 25453472	31121	4.9	8	119
6840	tubulin beta	gi | 223429	50223	4.67	6	79
7335	nuclear chloride channel protein	gi | 4588526	27249	5.02	10	54
7049	glyceraldehyde-3-phosphate dehydrogenase	gi | 31645	36202	8.26	8	143
6946	F-actin capping protein alpha-1 subunit	gi | 5453597	32923	5.45	9	84
6852	hypothetical protein	gi | 51476996	40696	6.43	7	68
7137	guanine nucleotide binding protein beta polypeptide 2-like 1	gi | 21619296	35077	7.6	6	125