A predictive factor for the response to S-1 plus cisplatin in gastric cancer

Table 1 Patient characteristics

Sex	Age (yr)	Performance status	Site	Size (cm)	Pathology	Metastasis
Male	62	1	Antrum	5	Diffuse	Peritoneum
Female	52	0	Fundus	4	Diffuse	Ovary
Female	67	2	Corpus	10	Diffuse	Lymph node
Male	68	2	Corpus	8	Intestinal	Lymph node
Male	78	2	Corpus	15	Intestinal	Lymph node
Male	64	2	Corpus	8	Intestinal	Liver
Male	67	2	Fundus	5	Intestinal	Liver
Female	70	2	Corpus	5	Diffuse	Peritoneum
Male	65	1	Corpus	4	Diffuse	Peritoneum
Female	59	2	Corpus	15	Diffuse	Peritoneum
Male	67	2	Corpus	5	Diffuse	Liver
Male	39	2	Corpus	20	Diffuse	Peritoneum
Female	62	0	Antrum	7	Intestinal	Liver
Male	77	1	Antrum	3	Intestinal	Liver

Table 2 Protein expression of thymidylate synthase, dihydropyrimidine dehydrogenase and orotate phosphoribosyl transferase in tumour tissues and matched normal tissues (ng/mg protein)

	No. of cases	Tumour tissue	Normal tissue	Paired t-test
TS	12	42.9 ± 19.9 (median 40.5)	21.5 ± 13.7 (median 19.9)	P < 0.001
DPD	14	156.6 ± 63.3 (median 143.3)	168.1 ± 36.5 (median 170.3)	NS
OPRT	12	9.3 ± 5.8 (median 8.7)	10.1 ± 6.1 (median 8.2)	NS

TS: Thymidylate synthase; DPD: Dihydropyrimidine dehydrogenase; OPRT: Orotate phosphoribosyl transferase; NS: Not significant.

Table 3 Protein expression of thymidylate synthase, dihydropyrimidine dehydrogenase and orotate phosphoribosyl transferase in relation to responder or non-responder on the first S-1/CDDP therapy (ng/mg protein)

	Responder (n = 6)	Non-reponder (n = 4)	t-test
TS	27.4 ± 9.4 (median 29.2)	56.9 ± 19.9 (median 53.6)	P < 0.05
DPD	170.9 ± 91.4 (median 135.7)	148.9 ± 33.5 (median 155.6)	NS
OPRT	6.1 ± 3.7 (median 6.5)	9.6 ± 9.7 (median 5.8)	NS

TS: Thymidylate synthase; DPD: Dihydropyrimidine dehydrogenase; OPRT: Orotate phosphoribosyl transferase; NS: Not significant.

Table 4 Correlation between changes of protein expression of thymidylate synthase, dihydropyrimidine dehydrogenase and orotate phosphoribosyl transferase and responses (ng/mg protein)

	1st evaluation	2nd evaluation	Paired t-test
Responder(1st evaluation) → responder (2nd evaluation) (n = 2)
TS	30.0 ± 2.8	19.2 ± 2.3	NS
DPD	161.7 ± 53.3	172.6 ± 22.8	NS
OPRT	9.8 ± 0.6	4.7 ± 1.7	NS
Responder(1st evaluation) → non-responder(2nd evaluation) (n = 3)
TS	31.7 ± 4.6	53.9 ± 3.9	P < 0.01
DPD	204.4 ± 119.5	279.1 ± 124.7	P < 0.05
OPRT	4.3 ± 3.1	5.3 ± 1.3	NS
Non-responder(1st evaluation) → non-responder (2nd evaluation) (n = 2)
TS	73.4 ± 10.7	77.3 ± 45.4	NS
DPD	142.3 ± 52.6	247.5 ± 60.5	P < 0.05
OPRT	5.9 ± 1.3	8.4 ± 1.9	NS

TS: Thymidylate synthase; DPD: Dihydropyrimidine dehydrogenase; OPRT: Orotate phosphoribosyl transferase; NS: Not significant.