Establishment of a human hepatoma multidrug resistant cell line in vitro

doi:10.3748/wjg.v16.i18.2291

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May 14, 2010 (publication date) through Dec 19, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. May 14, 2010; 16(18): 2291-2297
Published online May 14, 2010. doi: 10.3748/wjg.v16.i18.2291

Table 1 IC₅₀ and RI values of SK-Hep-1 and SK-Hep-1/CDDP cells

Drug (μg/mL)	IC₅₀ (mean ± SD)		RI
Drug (μg/mL)	SK-Hep-1	SK-Hep-1/CDDP	RI
CDDP	5.13 ± 0.09	70.61 ± 1.06^b	13.76
DOX	0.74 ± 0.04	4.13 ± 0.23^b	5.58
VCR	0.76 ± 0.02	2.28 ± 1.06^b	3.12
5-FU	12.49 ± 0.27	52.79 ± 3.85^b	4.23

^bSignificant difference in comparison with the parental cells (P < 0.01). SK-Hep-1 and SK-Hep-1/CDDP cells were exposed to indicated concentrations of CDDP, DOX, VCR and 5-FU for 24 h and determined using the CCK-8 assay. The IC₅₀ values were calculated. Values of 3 independent experiments are represented as mean ± SD. Resistance index (RI) = (IC₅₀ SK-Hep-1/CDDP cells)/(IC₅₀ SK-Hep-1 cells). CDDP: Cisplatin; IC₅₀: 50% inhibitory dose; DOX: Doxorubicin; VCR: Vincristine; 5-FU: 5-fluorouracil.

Table 2 Cell cycle distribution of SK-Hep-1 and SK-Hep-1/CDDP cells (%)

Cell	G0/G1	S	G2/M
SK-Hep-1	59.83 ± 3.28	27.91 ± 2.16	12.14 ± 3.36
SK-Hep-1/CDDP	37.50 ± 5.05^a	42.20 ± 2.65^a	20.67 ± 5.69^a

^aP < 0.05, resistant cells vs SK-Hep-1. SK-Hep-1/CDDP in G0/G1 phase decreased accompanied by an increased proportion of cells in the S phase and G2/M phase.

Table 3 Semi-quantification of P-gp and MRP1 immunofluorescence (mean ± SD)

Cell	P-gp (n = 3)	MRP1 (n = 3)
SK-Hep-1	20.57 ± 6.59	3.75 ± 0.02
SK-Hep-1/CDDP	64.79 ± 12.4^a	35.78 ± 6.17^a

^aP < 0.05, resistant cells vs SK-Hep-1.

Citation: Zhou Y, Ling XL, Li SW, Li XQ, Yan B. Establishment of a human hepatoma multidrug resistant cell line in vitro. World J Gastroenterol 2010; 16(18): 2291-2297
URL: https://www.wjgnet.com/1007-9327/full/v16/i18/2291.htm
DOI: https://dx.doi.org/10.3748/wjg.v16.i18.2291