LOH analysis of genes around D4S2964 identifies ARD1B as a prognostic predictor of hepatocellular carcinoma

Table 1 Clinicopathologic correlation of FAL index around D4S2964

Parameter	FAL index
	Low	High	P value
Sex
Female	9	9	1.000
Male	47	47
Serum HBsAg
Negative	9	7	0.589
Positive	47	49
Cirrhosis
Absent	10	2	0.021
Present	42	46
Tumor size (cm)
≤ 5	12	13	0.820
> 5	44	43
Tumor stage
I and II	44	41	0.608
III and IV	11	13
Recurrence
No	39	36	0.547
Yes	17	20

FAL: Fractional allelic loss.

Table 2 Representative clinicopathologic correlation of LOH in different genes around D4S2964

Parameter	LOH
	Absent	Present	P value
Tumor size (cm)	ART3a
≤ 5	16	3	0.043
> 5	39	27
Tumor size (cm)	NUP54
≤ 5	15	6	0.019
> 5	30	41
Tumor size (cm)	SCARB2
≤ 5	18	3	0.001
> 5	33	39
Tumor size (cm)	CCDC158
≤ 5	11	3	0.037
> 5	19	22
Serum HBeAg	SEPT11
Negative	50	32	0.041
Positive	4	9
Serum HBsAg	CCNI
Negative	11	1	0.037
Positive	48	31
Serum HBeAg	CXCL13
Negative	50	37	0.024
Positive	2	8
Recurrence
No	40	26	0.044
Yes	12	19
Tumor stage	ANXA3
I and II	37	29	0.014
III and IV	15	2
Serum HBV-DNA	ARD1B
Negative	24	5	0.049
Positive	14	10
Recurrence
No	32	9	0.046
Yes	7	7
Serum HBsAg	SEC31A
Negative	4	10	0.013
Positive	47	26
Metastasis
No	40	34	0.039
Yes	11	2
Cirrhosis	HELQ
Absent	8	0	0.006
Present	32	34
Cirrhosis	AGPAT9
Absent	7	3	0.033
Present	27	50

^aGenes are listed in order of the most centromeric (top) to the most telomeric (bottom). LOH: Loss of heterozygosity; HBV: Hepatitis B virus; ART3: ADP-ribosyltransferase 3; NUP54: Nucleoporin 54 kDa; SCARB2: Scavenger receptor class B, member 2; CCDC158: Coiled-coil domain containing 158; SEPT11: Septin 11; CCNI: Cyclin I; CXCL13: Chemokine (C-X-C motif) ligand 13; ANXA3: Annexin A3; SEC31A: SEC31 homolog A; HELQ: Helicase, POLQ-like; AGPAT9: 1-acylglycerol-3-phosphate O-acyltransferase 9.

Table 3 Univariate analyses of individual parameters for correlations with overall survival rate: Cox proportional hazards model

Parameter	Hazard ratio	95% CI	P value
LOH in ARD1B	2.915	1.119-7.594	0.029
LOH in SEPT11	2.856	1.354-6.023	0.006
Sex	1.327	0.518-3.398	0.556
Tumor grade	1.693	0.948-3.023	0.075
Serum HBsAg	0.902	0.377-2.158	0.817
Serum AFP	3.319	1.295-8.504	0.012
Tumor size	4.266	1.31-13.888	0.016
No. of nodules	3.143	1.627-6.073	0.001
Cirrhosis	0.937	0.329-2.673	0.903
Metastasis	1.736	0.764-3.946	0.188
Recurrence	1.992	1.049-3.783	0.035

Table 4 Multivariate analyses of individual parameters for correlations with overall survival rate: Cox proportional hazards model

Parameter	ARD1B			SEPT11			ARD1B and SEPT11a
	HR	95% CI	P value	HR	95% CI	P value	HR	95% CI	P value
LOH in ARD1B	4.615	1.538-13.851	0.006				4.866	1.528-15.493	0.007
LOH in SEPT11				2.393	1.107-5.170	0.026			0.533
Serum AFP	1.781	0.468-6.774	0.397	2.166	0.748-6.274	0.154			0.208
Tumor size	2.146	0.459-10.033	0.332	3.316	1.001-10.984	0.050			0.393
No. of nodules	6.173	2.072-18.394	0.001	3.002	1.401-6.434	0.005	5.579	1.738-17.904	0.004
Recurrence	0.900	0.305-2.659	0.849	1.707	0.829-3.516	0.147			0.859

^aForward LR method was used. HR: Hazard radio.