Natural taurine promotes apoptosis of human hepatic stellate cells in proteomics analysis

Table 1 MS identification of differentially expressed proteins in taurine-treated and control groups

Code	Protein spot ID	Record number of protein	Name of protein	Description	log2 (differential ratio)	Relative molecular weight (kDa)	Isoelectric point	Score	Coverage (%)
Up-regulated proteins
1	1394	gi|2392338	GLO1	Chain a, human glyoxalase i with benzyl-glutathione inhibitor	4.25	20.6	5.12	408	51
2	1431	gi|29568111	MYL9	Myosin regulatory light chain 9 isoform a	3.30	19.8	4.80	631	62
3	1330	gi|4506217	PSMD10	Proteasome 26S non-ATPase subunit 10 isoform 1	3.24	24.4	5.71	279	35
4	1380	gi|558528	PSMB6	Proteasome subunit Y	2.62	25.3	4.80	403	26
5	1438	gi|4507669	TPT1	Tumor protein, translationally-controlled 1	2.48	19.6	4.84	375	61
6	1428	gi|4502101	ANXA1	Annexin I	1.48	38.7	6.57	1069	53
7	1419	gi|5174539	MDH1	Cytosolic malate dehydrogenase	1.18	36.4	6.91	223	17
8	1321	gi|4504517	HSPB1	Heat shock protein beta-1	0.99	22.8	5.98	567	49
9	1432	gi|5453710	LASP1	LIM and SH3 protein 1	0.95	29.7	6.61	362	34
10	1385	gi|31543380	PARK7	Parkinson disease protein 7	0.70	19.9	6.33	128	50
11	1285	gi|2982080	SOD1	Chain a, familial als mutant g37r cuznsod	0.50	15.9	5.87	292	45
Down-regulated proteins
12	1130	gi|5453559	ATP5H	ATP synthase, H+ transporting, Mitochondrial F0 complex, subunit d isoform a	-0.66	18.5	5.21	277	47
13	1355	gi|5802974	PRDX3	Peroxiredoxin 3 isoform a precursor	-0.70	27.7	7.67	128	16
14	1284	gi|194097323	ECHS1	Mitochondrial short-chain enoyl-coenzyme A hydratase 1 precursor	-0.83	31.4	8.34	481	30
15	1067	gi|9955007	PRDX2	Chain a, thioredoxin peroxidase b from red blood cells	-1.14	21.8	5.44	446	38
16	953	gi|14043072	HNRNPA2B1	Heterogeneous nuclear ribonucleoprotein A2/B1 isoform B1	-1.86	37.4	8.97	181	15
17	1165	gi|190200	VDAC2	Porin	-2.81	38.1	6.32	281	22
18	1464	gi|1568551	HIST3H2BB	Histone H2B	-3.19	13.9	10.31	270	35
19	980	gi|5174391	AKR1A1	Aldo-keto reductase family 1, member A1	-3.55	36.6	6.32	306	22

MS: Mass spectrometry.

Table 2 Functional categorization of differentially expressed proteins

GO number	Functional description	Proportion (%)	Protein	P value	FDR value
Apoptosis-related
51093	Negative regulation of developmental process	32	TPT1|ANXA1|HSPB1|GLO1|PRDX2|SOD1	2.20E-6	2.61E-4
42981	Regulation of apoptosis	32	TPT1|ANXA1|HSPB1|GLO1|PRDX2|SOD1	3.19E-5	1.49E-3
43067	Regulation of programmed cell death	32	TPT1|ANXA1|HSPB1|GLO1|PRDX2|SOD1	3.45E-5	1.49E-3
Oxidation reaction-related
42542	Response to hydrogen peroxide	16	PRDX3|SOD1|PARK7	4.28E-6	4.07E-4
6979	Response to oxidative stress	21	PRDX2|PRDX3|SOD1|PARK7	8.18E-6	5.65E-4
302	Response to reactive oxygen species	16	PRDX3|SOD1|PARK7	8.33E-6	5.65E-4
Metabolism-related
44248	Cellular catabolic process	37	PSMB6|AKR1A1|PSMD10|ECHS1|PRDX3|SOD1|MDH1	9.67E-6	5.74E-4
9056	Catabolic process	37	PSMB6|AKR1A1|PSMD10|ECHS1|PRDX3|SOD1|MDH1	3.12E-5	1.49E-3
42743	Hydrogen peroxide metabolic process	11	PRDX3|SOD1	2.45E-4	9.69E-3

Hypergeometric test, FDR < 0.01. GO: Gene ontology.