Original Articles
Copyright ©2009 The WJG Press and Baishideng.
World J Gastroenterol. Feb 28, 2009; 15(8): 919-926
Published online Feb 28, 2009. doi: 10.3748/wjg.15.919
Table 1 Primers and probes used in real-time quantitative PCR
Probe or primer typeSequence (5'-3')
hopA FATCATGCTAGTGATGGCGTTAAAG
hopA RCAGGCATAGACGGAGGCAAT
hopA ProbeCCAAAAATATTGCATGCGTTCCCGC
hopB FCTTGGTGCAAAACATCGTCAAT
hopB RCCCGCCATAGCTCACTTGAT
hopB ProbeTAACGCTAGCCAACAACGCTAACATCAGCA
hopC FCGCTCTTTATAACGCGCAAGTAA
hopC RGCTGTTCCCGGCTCTGAAT
hopC ProbeTGGATAAAATCAACGCGCTCAACAATCAG
hopD FCTGCTTGAGCGCGGTTTAA
hopD RCAACCTAGACACTGGGAAAGCAT
hopD ProbeCTTGCGCTCTAGCGTTAGCGAACATGC
hopE FGGATTGCACAGGGAGTGTTGT
hopE RGCCCCATTAGCGTATTTAGCAT
hopE ProbeTTGCCCCCCAGGTCTTACCGCT
hefA FAGGGCGTTTTGGGAATTTCT
hefA RGCATGATGGATTGTTTTTGCA
hefA ProbeCCCCGGTCAGCAAAATACGGCTG
hefB FAGGGCGATGTTTTGTTGCTT
hefB ICCCCCAATTTTGCTGTATCGT
hefB ProbeAATCAAGACAAACAGGCTCAAAGCGATTCC
hefC FGTTTGCGTTCTTGCGTAACG
hefC RTGTTTAATGAAAAGCCCATCCA
hefC ProbeCACGATCACCCTCGTTTCAGCGATC
16S rRNA FCCGCCTACGCGCTCTTTAC
16S rRNA RCTAACGAATAAGCACCGGCTAAC
16S rRNA ProbeCCCAGTGATTCCGAGTAACGCTTGCA
Table 2 cDNAs levels of porins and efflux system of H pylori tested by real-time quantitative PCR
Levels of cDNA
GeneAspirin groupDMSO controlBlank control
hopA963.60 ± 23.331087.75 ± 26.42856.27 ± 25.43
hopB84.00 ± 15.9791.45 ± 14.83100.15 ± 16.28
hopC52.98 ± 11.6751.77 ± 12.0152.79 ± 10.27
hopD83.86 ± 14.0992.46 ± 17.7889.92 ± 13.72
hopE54.07 ± 10.5852.77 ± 11.9352.12 ± 9.92
hefA12.31 ± 2.1212.51 ± 1.9912.97 ± 2.76
hefB25.47 ± 4.7328.48 ± 5.0725.60 ± 4.99
hefC55.62 ± 8.8952.08 ± 9.7853.37 ± 10.04