Insulin-like growth factor binding protein-7 induces activation and transdifferentiation of hepatic stellate cells in vitro

Table 1 Immunocytochemistry staining and Western blotting analysis of IGFBPrP1 (mean ± SD)

	n	Immunohistochemistry (The value of IOD × 105)	n	Western blotting (The value of A× 105)
Control	6	3.70 ± 0.32	6	0.03 ± 0.01
TGFβ1 2 ng/mL	6	4.86 ± 0.27a	6	1.05 ± 0.03a
TGFβ1 4 ng/mL	6	6.45 ± 0.32a	6	1.16 ± 0.02a
TGFβ1 8 ng/mL	6	4.91 ± 0.30a	6	1.01 ± 0.02a
TGFβ1 16 ng/mL	6	4.31 ± 0.26a	6	1.06 ± 0.02a

IOD: Integrated optical density; A: Absorbance; IGFBP: Insulin-like growth factor binding proteins;

^aP < 0.05 vs control group.

Table 2 ELISA determination of collagen I (mean ± SD)

	n	Collagen I (ng/mL)
Control	6	36.61 ± 1.28
TGFβ1 2 ng/mL	6	41.93 ± 2.95c
TGFβ1 4 ng/mL	6	49.44 ± 3.21c
Anti-IGFBPrP1 antibody 0.1 &mgr;g/mL	6	35.46 ± 5.80a
Anti-IGFBPrP1 antibody 1 &mgr;g/mL	6	36.38 ± 2.59a
Anti-IGFBPrP1 antibody 2 &mgr;g/mL	6	37.26 ± 2.12a
TGFβ1 2 ng/mL + Anti-IGFBPrP1	6	38.24 ± 2.76c
antibody 0.1 &mgr;g/mL
TGFβ1 2 ng/mL + Anti-IGFBPrP1	6	33.95 ± 3.27c
antibody 1 &mgr;g/mL
TGFβ1 2 ng/mL + Anti-IGFBPrP1	6	34.59 ± 3.97c
antibody 2 &mgr;g/mL
TGFβ1 4 ng/mL + Anti-IGFBPrP1	6	40.24 ± 3.46e
antibody 0.1 &mgr;g/mL
TGFβ1 4 ng/mL + Anti-IGFBPrP1	6	32.61 ± 4.86e
antibody 1 &mgr;g/mL

Relative expression of collagen I was measured by ELISA; after HSC incubated for 24 h, supernatant was obtained and analyzed. Statistical analysis was done by the SNK-q test.

^aP < 0.05, compared with the control group;

^cP < 0.05, compared with the TGFβ1 2 ng/mL;

^eP < 0.05, compared with TGF-β1 4 ng/mL.