Hepatoprotective activity of Sapindus mukorossi and Rheum emodi extracts: In vitro and in vivo studies

doi:10.3748/wjg.14.2566

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 14, Issue 16

This Article

Citation of this article

Corresponding Author of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (3485)

All Articles published online

The chart showing PDF series, HTML series, Figures (1-1) series, Tables (1-3) series.

Item

Count

PDF

383

HTML

2817

Figures (1-1)

137

Tables (1-3)

148

Sum=3485

Apr 28, 2008 (publication date) through May 7, 2024

Times Cited of This Article

Times Cited (49)

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Rapid Communication

World J Gastroenterol. Apr 28, 2008; 14(16): 2566-2571
Published online Apr 28, 2008. doi: 10.3748/wjg.14.2566

Table 1 In vitro cytotoxicity profile of extracts of S. mukorossi and R. emodi (mean ± SE)

Sample identity	Test concen (&mgr;g/mL)	Cytotoxicity (% release of enzyme in the medium)
		LDH		GPT
		Sp. activity	%leakage	Sp. activity	%leakage
Control	-	5.05 ± 0.22	-	103 ± 2.5	-
S. mukorossi	10	5.00 ± 2.21	NS	111 ± 2.4	NS
	50	4.97 ± 0.23	NS	108 ± 3.1	NS
	100	5.18 ± 0.31	NS	100 ± 1.9	NS
R. emodi	10	5.03 ± 0.11	NS	119 ± 2.0	NS
	50	5.12 ± 0.17	NS	107 ± 2.5	NS
	100	5.08 ± 0.14	NS	114 ± 2.3	NS

NS: Non-significant (t-test).

Table 2 In vitro hepatoprotective activity of S. mukorossi and R. emodi extracts (mean ± SE)

Sample identity	Test concen (&mgr;g/mL)	Hepatoprotective effect
		GSH levels (cells)		[% restoration of enzyme leakage against toxin challenge (CCl₄) of enzyme leakage]
		&mgr;g/3 x 10⁶ cells	% recovery	LDH		GPT
		&mgr;g/3 x 10⁶ cells	% recovery	Sp. activity	% restoration	Sp. activity	% restoration
Control	-	5.61 ± 0.13	-	3.22 ± 0.25	100	94 ± 3.0	0
CCl₄	2.5 mmol/L	2.52 ± 0.14	-	16.0 ± 0.22	0	198 ± 2.5	0
CCl₄ +	10 &mgr;g	2.95 ± 0.22	16	10.2 ± 2.21^a	46	135 ± 2.4^a	60
S. mukorossi	50 &mgr;g	3.05 ± 0.17	20^a	8.10 ± 0.23^b	61	123 ± 3.1^a	72
	100 &mgr;g	3.31 ± 0.19	34^a	6.80 ± 0.31^b	71	105 ± 1.9^b	89
CCl₄ +	10 &mgr;g	3.12 ± 0.13	24^a	10.51 ± 0.11^a	43	143 ± 2.0^a	52
R. emodi	50 &mgr;g	3.27 ± 0.21	32^a	7.34 ± 0.17^b	67	129 ± 2.5^a	66
	100 &mgr;g	3.30 ± 0.13	33^a	6.08 ± 0.14^b	77	108 ± 2.3^a	86
CCl₄ + Silymarin	30 &mgr;g	4.61 ± 0.22	69^a	6.00 ± 0.50^b	78	107 ± 2.3^a	87

^aP < 0.05,

^bP < 0.01 vs control.

Table 3 Effect of S. mukorossi and R. emodi extracts on serum biochemical parameters against CCl₄ induced hepatic injury in rats (Curative study, mean ± SE)

Treatment	*Dose (mg/kg, p.o.)*	Serum parameters
Treatment	*Dose (mg/kg, p.o.)*	ALT (IU/L)	AST (IU/L)	ALP (IU/L)	Bilirubin (mg)
Vehicle control	-	127.73 ± 10.65	100.26 ± 11.50	40.11 ± 2.20	0.11 ± 0.02
Vehicle + CCl₄	-	1142.36 ± 80.97	833.59 ± 34.85	64.06 ± 3.00	0.82 ± 0.08
S. mukorossi CCl₄ +	2.5	524.18 ± 86.30^b	408.72 ± 40.07^a	47.38 ± 1.02^b	0.40 ± 0.02^b
R. emodi CCl₄ +	3	384.69 ± 44.39^b	314.90 ± 44.41^b	49.00 ± 2.19	0.32 ± 0.02^b
Silymarin CCl₄ +	50	563.47 ± 49.22^b	421.54 ± 30.82^b	47.96 ± 2.49^b	0.42 ± 0.02^b

^aP < 0.05,

^bP < 0.01 vs control.

Citation: Ibrahim M, Khaja MN, Aara A, Khan AA, Habeeb MA, Devi YP, Narasu ML, Habibullah CM. Hepatoprotective activity of Sapindus mukorossi and Rheum emodi extracts: In vitro and in vivo studies. World J Gastroenterol 2008; 14(16): 2566-2571
URL: https://www.wjgnet.com/1007-9327/full/v14/i16/2566.htm
DOI: https://dx.doi.org/10.3748/wjg.14.2566