Ibrahim M, Khaja MN, Aara A, Khan AA, Habeeb MA, Devi YP, Narasu ML, Habibullah CM. Hepatoprotective activity of Sapindus mukorossi and Rheum emodi extracts: In vitro and in vivo studies. World J Gastroenterol 2008; 14(16): 2566-2571 [PMID: 18442207 DOI: 10.3748/wjg.14.2566]
Corresponding Author of This Article
Mohammed Ibrahim, Nizam Institute of Pharmacy, Deshmukhi, pochampally (Mandal), Near Ramoji Film City, Nalgonda-508284, Andhra Pradesh, India. ibrahim_cce@rediffmail.com
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Rapid Communication
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World J Gastroenterol. Apr 28, 2008; 14(16): 2566-2571 Published online Apr 28, 2008. doi: 10.3748/wjg.14.2566
Hepatoprotective activity of Sapindus mukorossi and Rheum emodi extracts: In vitro and in vivo studies
Mohammed Ibrahim, Mohammed Nane Khaja, Anjum Aara, Aleem Ahmed Khan, Mohammed Aejaz Habeeb, Yalavarthy Prameela Devi, Mangamoori Lakshmi Narasu, Chitoor Mohammed Habibullah
Mohammed Ibrahim, Mohammed Nane Khaja, Aleem Ahmed Khan, Mohammed Aejaz Habeeb, Chitoor Mohammed Habibullah, Centre for Liver Research and Diagnostics, Deccan college of Medical Sciences and Allied Hospitals, Kanchanbagh, Hyderabad 500058, Andhra Pradesh, India
Mohammed Ibrahim, Anjum Aara, Nizam Institute of Pharmacy, Deshmukhi, pochampally (Mandal), Near Ramoji Film City, Nalgonda 508284, Andhra Pradesh, India
Yalavarthy Prameela Devi, Mangamoori Lakshmi Narasu, Centre for Biotechnology, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad 500072, Andhra Pradesh, India
Author contributions: Ibrahim M and Anjum A wrote the paper and organized the figures; Khaja MN, Aleem AK, and Habeeb MA helped write, organize, and correct the paper, the statistical data were carried out by Prameela DY and Lakshminarasu M, Habibullah CM supervised the writing and organization process.
Correspondence to: Mohammed Ibrahim, Nizam Institute of Pharmacy, Deshmukhi, pochampally (Mandal), Near Ramoji Film City, Nalgonda-508284, Andhra Pradesh, India. ibrahim_cce@rediffmail.com
Telephone: +91-40-24342954
Fax: +91-40-24342954
Received: October 2, 2007 Revised: March 4, 2008 Published online: April 28, 2008
Abstract
AIM: To study the hepatoprotective capacity of Sapindus mukorossi (S. mukorossi) and Rheum emodi (R. emodi) extracts in CCl4 treated male rats.
METHODS: The dried powder of S. mukorossi and R. emodi was extracted successively with petroleum ether, benzene, chloroform, and ethanol and concentrated in vacuum. Primary rat hepatocyte monolayer cultures were used for in vitro studies. In vivo, the hepatoprotective capacity of the extract of the fruit pericarp of S. mukorossi and the rhizomes of R. emodi was analyzed in liver injured CCl4-treated male rats.
RESULTS: In vitro: primary hepatocytes monolayer cultures were treated with CCl4 and extracts of S. mukorossi & R. emodi. A protective activity could be demonstrated in the CCl4 damaged primary monolayer culture. In vivo: extracts of the fruit pericarp of S. mukorossi (2.5 mg/mL) and rhizomes of R. emodi (3.0 mg/mL) were found to have protective properties in rats with CCl4 induced liver damage as judged from serum marker enzyme activities.
CONCLUSION: The extracts of S. mukorossi and R. emodi do have a protective capacity both in vitro on primary hepatocytes cultures and in in vivo in a rat model of CCl4 mediated liver injury.
