Immunohistochemical analysis of p53, cyclinD1, RB1, c-fos and N-ras gene expression in hepatocellular carcinoma in Iran

Table 1 Expression of p53 gene in relation to the expression of RB1, cyclinD1, c-fos and N-ras genes, n (%)

Geneexpression	RB1		Cyclin-D1		c-fos		N-ras
	Positive	Negative	Positive	Negative	Positive	Negative	Positive	Negative
P53 positive	2 (33.3)	4 (66.7)	2 (33.3)	4 (66.7)	4 (66.7)	2 (33.3)	1 (16.75)	5 (83.3)
P53 negative	1 (5.3)	18 (94.7)	3 (15.8)	16 (84.2)	8 (42.1)	11 (57.9)	1 (5.3)	18 (94.7)
	OR: 0.1, 95% CI: 0.0-1.5		OR: 2.6, 95% CI: 0.3-21.7		OR: 2.7, 95% CI: 0.4-18.8		OR: 3.6, 95% CI: 0.1-68.3

Table 2 RB1 gene expression in relation to the expression of c-fos and N-ras genes, n (%)

Geneexpression	c-fos		N-ras
	Positive	Negative	Positive	Negative
RB1 positive	1 (33.3)	2 (66.7)	0	3 (100)
RB1 negative	11 (50)	11 (50)	2 (9.1)	20 (90.9)
	OR: 0.5, 95% CI: 0.0-6.3		OR: 1.1, 95% CI : 0.9-1.2

Table 3 G1 checkpoint protein expression in relation to p53, n (%)

Expression pattern of G1checkpoint proteins	P53 (+)	P53 (-)	P
Rb+/cyclinD1+	Zero	Zero
Rb-/cyclinD1+	2 (40)	3 (60)
Rb-/cyclinD1-	2 (11.8)	15 (88.2)	0.02
Subtotal	4 (66.6)	18 (94.7)
Rb+/cyclin D1-	2 (66.7)	1 (33.3)
Total	6 (24)	19 (76)