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World J Gastroenterol. Jan 28, 2007; 13(4): 588-593
Published online Jan 28, 2007. doi: 10.3748/wjg.v13.i4.588
Immunohistochemical analysis of p53, cyclinD1, RB1, c-fos and N-ras gene expression in hepatocellular carcinoma in Iran
SJ Moghaddam, EN Haghighi, S Samiee, N Shahid, AR Keramati, S Dadgar, MR Zali
SJ Moghaddam, EN Haghighi, S Samiee, N Shahid, AR Keramati, S Dadgar, MR Zali, Research Center for Gastroenterology and Liver Disease, Shaheed Beheshti University of Medical Sciences, and Academy of Medical Sciences, Iran
Author contributions: All authors contributed equally to the work.
Supported by Academy of Medical Sciences, Iran
Correspondence to: Mohammad Reza Zali, MD, FACG, University Professor in Shahid Beheshti University of Medical Sciences, President of Research Center for Gastroenterology and Liver Disease, 7th floor, Taleghani Hospital Tabnak St., Evin,Tehran 19857, Iran. zali@rcgld.org
Telephone: +98-21-22418871 Fax: +98-21-22402639
Received: July 13, 2006
Revised: July 29, 2006
Accepted: November 27, 2006
Published online: January 28, 2007
Abstract

AIM: To study the effect of some genes especially those involved in cell cycle regulation on hepatocellular carcinoma.

METHODS: Paraffin-embedded tissue samples of 25 patients (18 males and 7 females) with hepatocellular carcinoma were collected from 22 pathology centers in Tehran during 2000-2001, and stained using immunohistochemistry method (avidin-biotin-peroxidase) for detection of p53, cyclinD1, RB1, c-fos and N-ras proteins.

RESULTS: Six (24%), 5 (20%), 12 (48%) and 2 samples (8%) were positive for p53, cyclinD1, C-fos and N-ras expression, respectively. Twenty-two (88%) samples had alterations in the G1 cell-cycle checkpoint protein expression (RB1 or cyclinD1). P53 positive samples showed a higher (9 times) risk of being positive for RB1 protein than p53 negative samples. Loss of expression of RB1 in association with p53 over-expression was observed in 4 (66.7%) of 6 samples. Loss of expression of RB1 was seen in all cyclinD1 positive, 20 (90.9%) N-ras negative, and 11 (50%) C-fos positive samples, respectively. CyclinD1 positive samples showed a higher (2.85 and 4.75 times) risk of being positive for c-fos and N-ras expression than cyclinD1 negative samples.

CONCLUSION: The expression of p53, RB1 and c-fos genes appears to have a key role in the pathogenesis of hepatocellular carcinoma in Iran. Simultaneous overexpression of these genes is significantly associated with their loss of expression during development of hepatocellular carcinoma.

Keywords: Hepatocellular Carcinoma; Iran; Expression of p53, cyclinD1, RB1, c-fos and N-ras genes