Matrix metalloproteinase-9-1562C>T polymorphism may increase the risk of lymphatic metastasis of colorectal cancer

Table 1 Characteristics of cases and controls

	Controls/cases	OR	95% CI
Sex
Male	101/70	1
Female	98/67	0.986	(0.638-1.524)
Age (yr)
≤ 60	98/60	1
> 60	101/77	1.245	(0.804-1.928)
Smoking status
Non-smoker	147/75	1
Smoker	52/62	2.337	(1.473-3.708)b
Alcohol duration (yr)
Never	168/103	1
1-15	16/14	1.427	(0.669-3.046)
>15	15/20	2.175	(1.066-4.437)
BMI (kg/m2)
18.5-22.9	101/35	1
23-24.9	38/54	4.101	(2.329-7.220)
> 25	60/48	2.309	(1.345-3.962)d

^bP < 0.001 vs non-smoker group (Two-sided χ² test);

^dP < 0.001 vs normal BMI group (Two-sided χ² test).

Table 2 MMP-9 genotypes and the risk of CRC n (%)

Genotypes	Controls	Cases	OR1 (95% CI)
C-1562T
CC	147 (73.9)	104 (75.9)	1.00
CT	47 (23.6)	33 (24.1)	0.877 (0.527-1.462)
TT	5 (2.5)	0 (0)
T allele	0.143	0.12
R279Q
QQ	16 (8.0)	12 (8.8)	1.00
RQ	85 (42.7)	58 (42.3)	0.936 (0.410-2.138)
RR	98 (49.2)	67 (48.9)	0.961 (0.424-2.177)
R allele	0.706	0.701
C-1562T and R279Q combinations
279 QQ and -1562CC	11 (5.5)	9 (6.6)	1.00
1-2 risk alleles2	155(77.9)	109 (79.6)	1.357 (0.474-3.886)
> 2 risk alleles	33 (16.6)	19 (13.9)	1.227 (0.661-2.277)

¹OR for CT/TT genotypes versus CC genotypes and adjusted for age, gender, smoking status, alcohol consumption and body mass index.

²Risk alleles were MMP-9 279R and -1562T.

Table 3 Relationship of the C-1562T and R279Q genotypes with clinicopathological features of CRC patients

Parameters	C-1562T		R279Q
	CC	CT+TT	QQ	RQ+RR
Age (yr)
< 60 (n = 60)	49	11	1	59
≥ 60 (n = 77)	55	22	11	66c
Sex
Male (n= 70)	57	13	6	64
Female (n = 67)	47	20	6	61
Lymph node metastasis
N(-)(n = 87)	71	16	8	79
N(+)(n = 46)	29	17a	4	42
TNM classification
StageI(n = 26)	21	5	3	23
≥ Stage II(n = 107)	79	28	9	98
External membrane invasion
(+) (n = 102)	76	26	9	93
(-) (n = 31)	24	7	3	28

^aP = 0.022 vs CC genotype;

^cP = 0.012 vs QQ genotype, Fisher’s exact test.