Evaluation of the effect of pyrrolidine dithiocarbamate in suppressing inflammation in mice with dextran sodium sulfate-induced colitis

Table 1 Disease activity index score

Score	Weight loss (%)	Stool consistency	Occult/grossbleeding
0	(-)	Normal	Normal
1	1-5
2	6-10	Loose	Guiac (+)
3	11-15
4	> 15	Diarrhea	Gross bleeding

The disease activity index = (combined score of weight loss, stool consistency and bleeding)/3. Normal stools = well formed pellets; Loose = pasty stool which do not stick to the anus; Diarrhea = liquid stools that stick to the anus.

Table 2 Histological disease score

Grade 0		Normal colonic mucosa
Grade 1		Loss of one-third of the crypts
Grade 2		Loss of two-third of the crypts
Grade 3		The lamina propria is covered with a single layer of epithelium and mild inflammatory cell infiltration is present
Grade 4		Erosions and marked inflammatory cell infiltration are present

Randomly selected 8 fields (magnified 100 times) in each section were inspected and graded as above by a pathologist who was blinded to the treatment protocol. The mean in each section was calculated by scoring the grades in 8 fields.