Th immune response induced by H pylori vaccine with chitosan as adjuvant and its relation to immune protection

doi:10.3748/wjg.v13.i10.1547

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Mar 14, 2007 (publication date) through Feb 5, 2025

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

H Pylori

World J Gastroenterol. Mar 14, 2007; 13(10): 1547-1553
Published online Mar 14, 2007. doi: 10.3748/wjg.v13.i10.1547

Table 1 Rates of immune protection of H pylori vaccine with different kinds of adjuvant

Groups	n	H pylori positiven (%)	Rates of immuneprotection (%)
(1) Control	10	10 (100)	0
(2) Chi-solution	12	12 (100)	0
(3) Chi-particles	13	13 (100)	0
(4) Hp antigen	10	10 (100)	0
(5) Hp antigen + CT	12	5 (41.67)	58.33^d
(6) Hp antigen + chi-solution	10	4 (40)	60^a^b
(7) Hp antigen + chi-particles	10	4 (40)	60^a^b
P < 0.001

^aP < 0.05 vs (1) and (4) groups;

^bP < 0.01 vs (2) and (3) groups;

^dP < 0.01 vs (1)-(4) groups; chi = chitoson.

Table 2 H pylori colonization score in gastric mucosa

Groups	n	H pylori colonization score
Groups	n	0	1	2	3
(1) Control	10	0	2	4	4
(2) Chi-solution^d	12	1	8	2	1
(3) Chi-particles^d	13	0	10	1	2
(4) Hp antigen^c	10	1	5	2	2
(5) Hp antigen + CT^a^d	12	7	2	1	2
(6) Hp antigen + chi-solution^b	10	6	4	0	0
(7) Hp antigen + chi-particles^b	10	7	3	0	0
H = 57.181, P < 0.001

^bP < 0.01 vs (1)-(4) groups;

^aP < 0.05 vs (4) group;

^cP < 0.05 vs (1) group;

^dP < 0.01 vs (1) group; chi = chitoson.

Table 3 Density of H pylori colonization in gastric mucosa

Groups	n	Median of H pyloricolony number (CFU/g)
(1) Control	10	4.74 × 10⁵
(2) Chi-solution	12	0.73 × 10⁵
(3) Chi-particles	13	1.83 × 10⁵
(4) Hp antigen	10	0.48 × 10⁵
(5) Hp antigen + CT	12	0
(6) Hp antigen + chi-solution	10	0
(7) Hp antigen + chi-particles	10	0
H = 27.43, P = 0.001

chi = chitoson.

Table 4 Levels of IL-2, IFN-γ, IL-12, IL-10 and IL-4 in gastric mucosa before challenge (mean ± SD)

Groups	n	IL-2	IFN-γ	IL-12	IL-10	IL-4
(1) Control	5	19.9 ± 12.6	28.6 ± 10.2	118.9 ± 46.2	67.2 ± 32.5	4.19 ± 2.95
(2) Hp antigen	5	20.6 ± 3.5	33.6 ± 13.7	165.3 ± 47.4	104.3 ± 19.1	6.49 ± 2.61
(3) Hp antigen chi-solution	5	28.6 ± 9.3	58.5 ± 12.2^b	283.5 ± 93.7^c^d	255.3 ± 131.8^c^d	14.70 ± 8.73^a
(4) Hp antigen+ chi-particles	5	27.6 ± 13.9	59.8 ± 15.2^b	283.4 ± 99.6^c^d	237.1 ± 98.3^c^d	14.48 ± 6.84^a
		F = 0.739	F = 7.948	F = 6.083	F = 6.228	F = 4.189
		P = 0.544	P = 0.002	P = 0.006	P = 0.005	P = 0.023

^aP < 0.05 vs (1) and (2) groups;

^bP < 0.01 vs (1) and (2) groups;

^cP < 0.05 vs (2) group;

^dP < 0.01 vs (1) group; chi = chitoson.

Table 5 Levels of IL-2, IFN-γ, IL-12, IL-10 and IL-4 in gastric mucosa after challenge (mean ± SD)

Groups	n	IL-2	IFN-γ	IL-12	IL-10	IL-4
(1) Control	10	34.3 ± 11.8	48.7 ± 27.8	233.7 ± 125.7	86.4 ± 38.2	3.87 ± 1.99
(2) Chi-solution	11	31.8 ± 27.4	56.8 ± 26.1	308.5 ± 178.7	100.7 ± 52.6	4.47 ± 1.89
(3) Chi-particles	10	51.1 ± 42.6	58.8 ± 28.1	311.3 ± 129.6	88.4 ± 51.7	7.21 ± 4.02^g
(4) Hp antigen	9	97.9 ± 64.2^a	59.4 ± 15.0	319.4 ± 136.3	89.3 ± 29.01	4.89 ± 3.15
(5) Hp antigen + CT	10	80.9 ± 60.2^c	93.6 ± 23.5^e^f	487.0 ± 289.3^e^f	81.8 ± 49.5	3.67 ± 1.76
(6) Hp antigen + chi-solution	10	124.3 ± 75.2^b	107.5 ± 42.0^d	525.6 ± 112.2^d	108.5 ± 39.0	8.78 ± 4.96^hⁱ
(7) Hp antigen + chi-particles	10	88.6 ± 57.0^a	105.9 ± 48.1^d	554.0 ± 164.4^d	93.1 ± 39.2	6.59 ± 1.38^k
		F = 3.370	F = 6.346	F = 5.448	F = 0.745	F = 3.214
		P = 0.002	P < 0.001	P < 0.001	P > 0.05	P = 0.003

^aP < 0.05 vs (1) and (2) groups;

^bP < 0.001 vs (1)-(3) groups;

^cP < 0.05 vs (1) group;

^dP < 0.01 vs (1)-(4) groups;

^eP < 0.05 vs (2)-(4) groups;

^fP < 0.01 vs (1) group;

^gP < 0.05 vs (1), (2) and (5) groups,

^hP < 0.01 vs (1), (2) and (5) groups;

ⁱP < 0.05 vs (4) group;

^kP < 0.05 vs (5) group; chi = chitoson.

Table 6 Levels of anti-HpIgG2a, IgG1 and ratio of IgG2a/IgG1 in serum after challenge (mean ± SD)

Groups	N	IgG2a	IgG1	IgG2a/IgG1
(1) Control	10	4.44 ± 2.09	2.77 ± 1.18	2.13 ± 1.24
(2) Chi-solution	11	6.28 ± 3.62	3.96 ± 2.32	1.64 ± 0.85
(3) Chi-particles	10	5.56 ± 2.27	4.57 ± 2.09	1.43 ± 0.37^g^h
(4) Hp antigen	9	9.21 ± 6.80^a	6.43 ± 4.88^a	2.356 ± 1
(5) Hp antigen + CT	10	11.22 ± 5.52^a	7.71 ± 5.11^d^e	2.19 ± 0.86
(6) Hp antigen + chi-solution	10	11.48 ± 5.55^a	9.04 ± 5.35^b	1.06 ± 0.4^f
(7) Hp antigen + chi-particles	10	16.13 ± 10.20^b	8.02 ± 5.29^c^d	1 ± 0.37^f
		F = 4.572	F = 10.308	F = 3.780
		P < 0.001	P < 0.001	P < 0.001

^aP < 0.05 vs (1) group;

^bP < 0.01 vs (1)-(3) groups;

^cP < 0.05 vs (2) and (3) groups,

^dP < 0.01 vs (1) group;

^eP < 0.05 vs (2) group;

^fP < 0.01 vs (1), (4) and (5) groups;

^gP < 0.05 vs (5) group;

^hP < 0.01 vs (4) group.

Citation: Xie Y, Zhou NJ, Gong YF, Zhou XJ, Chen J, Hu SJ, Lu NH, Hou XH. Th immune response induced by H pylori vaccine with chitosan as adjuvant and its relation to immune protection. World J Gastroenterol 2007; 13(10): 1547-1553
URL: https://www.wjgnet.com/1007-9327/full/v13/i10/1547.htm
DOI: https://dx.doi.org/10.3748/wjg.v13.i10.1547