Copyright
©2007 Baishideng Publishing Group Co.
World J Gastroenterol. Mar 14, 2007; 13(10): 1547-1553
Published online Mar 14, 2007. doi: 10.3748/wjg.v13.i10.1547
Published online Mar 14, 2007. doi: 10.3748/wjg.v13.i10.1547
Table 1 Rates of immune protection of H pylori vaccine with different kinds of adjuvant
Table 2 H pylori colonization score in gastric mucosa
Table 3 Density of H pylori colonization in gastric mucosa
Groups | n | Median of H pyloricolony number (CFU/g) |
(1) Control | 10 | 4.74 × 105 |
(2) Chi-solution | 12 | 0.73 × 105 |
(3) Chi-particles | 13 | 1.83 × 105 |
(4) Hp antigen | 10 | 0.48 × 105 |
(5) Hp antigen + CT | 12 | 0 |
(6) Hp antigen + chi-solution | 10 | 0 |
(7) Hp antigen + chi-particles | 10 | 0 |
H = 27.43, P = 0.001 |
Table 4 Levels of IL-2, IFN-γ, IL-12, IL-10 and IL-4 in gastric mucosa before challenge (mean ± SD)
Groups | n | IL-2 | IFN-γ | IL-12 | IL-10 | IL-4 |
(1) Control | 5 | 19.9 ± 12.6 | 28.6 ± 10.2 | 118.9 ± 46.2 | 67.2 ± 32.5 | 4.19 ± 2.95 |
(2) Hp antigen | 5 | 20.6 ± 3.5 | 33.6 ± 13.7 | 165.3 ± 47.4 | 104.3 ± 19.1 | 6.49 ± 2.61 |
(3) Hp antigen chi-solution | 5 | 28.6 ± 9.3 | 58.5 ± 12.2b | 283.5 ± 93.7cd | 255.3 ± 131.8cd | 14.70 ± 8.73a |
(4) Hp antigen+ chi-particles | 5 | 27.6 ± 13.9 | 59.8 ± 15.2b | 283.4 ± 99.6cd | 237.1 ± 98.3cd | 14.48 ± 6.84a |
F = 0.739 | F = 7.948 | F = 6.083 | F = 6.228 | F = 4.189 | ||
P = 0.544 | P = 0.002 | P = 0.006 | P = 0.005 | P = 0.023 |
Table 5 Levels of IL-2, IFN-γ, IL-12, IL-10 and IL-4 in gastric mucosa after challenge (mean ± SD)
Groups | n | IL-2 | IFN-γ | IL-12 | IL-10 | IL-4 |
(1) Control | 10 | 34.3 ± 11.8 | 48.7 ± 27.8 | 233.7 ± 125.7 | 86.4 ± 38.2 | 3.87 ± 1.99 |
(2) Chi-solution | 11 | 31.8 ± 27.4 | 56.8 ± 26.1 | 308.5 ± 178.7 | 100.7 ± 52.6 | 4.47 ± 1.89 |
(3) Chi-particles | 10 | 51.1 ± 42.6 | 58.8 ± 28.1 | 311.3 ± 129.6 | 88.4 ± 51.7 | 7.21 ± 4.02g |
(4) Hp antigen | 9 | 97.9 ± 64.2a | 59.4 ± 15.0 | 319.4 ± 136.3 | 89.3 ± 29.01 | 4.89 ± 3.15 |
(5) Hp antigen + CT | 10 | 80.9 ± 60.2c | 93.6 ± 23.5ef | 487.0 ± 289.3ef | 81.8 ± 49.5 | 3.67 ± 1.76 |
(6) Hp antigen + chi-solution | 10 | 124.3 ± 75.2b | 107.5 ± 42.0d | 525.6 ± 112.2d | 108.5 ± 39.0 | 8.78 ± 4.96hi |
(7) Hp antigen + chi-particles | 10 | 88.6 ± 57.0a | 105.9 ± 48.1d | 554.0 ± 164.4d | 93.1 ± 39.2 | 6.59 ± 1.38k |
F = 3.370 | F = 6.346 | F = 5.448 | F = 0.745 | F = 3.214 | ||
P = 0.002 | P < 0.001 | P < 0.001 | P > 0.05 | P = 0.003 |
Table 6 Levels of anti-HpIgG2a, IgG1 and ratio of IgG2a/IgG1 in serum after challenge (mean ± SD)
Groups | N | IgG2a | IgG1 | IgG2a/IgG1 |
(1) Control | 10 | 4.44 ± 2.09 | 2.77 ± 1.18 | 2.13 ± 1.24 |
(2) Chi-solution | 11 | 6.28 ± 3.62 | 3.96 ± 2.32 | 1.64 ± 0.85 |
(3) Chi-particles | 10 | 5.56 ± 2.27 | 4.57 ± 2.09 | 1.43 ± 0.37gh |
(4) Hp antigen | 9 | 9.21 ± 6.80a | 6.43 ± 4.88a | 2.356 ± 1 |
(5) Hp antigen + CT | 10 | 11.22 ± 5.52a | 7.71 ± 5.11de | 2.19 ± 0.86 |
(6) Hp antigen + chi-solution | 10 | 11.48 ± 5.55a | 9.04 ± 5.35b | 1.06 ± 0.4f |
(7) Hp antigen + chi-particles | 10 | 16.13 ± 10.20b | 8.02 ± 5.29cd | 1 ± 0.37f |
F = 4.572 | F = 10.308 | F = 3.780 | ||
P < 0.001 | P < 0.001 | P < 0.001 |
-
Citation: Xie Y, Zhou NJ, Gong YF, Zhou XJ, Chen J, Hu SJ, Lu NH, Hou XH. Th immune response induced by
H pylori vaccine with chitosan as adjuvant and its relation to immune protection. World J Gastroenterol 2007; 13(10): 1547-1553 - URL: https://www.wjgnet.com/1007-9327/full/v13/i10/1547.htm
- DOI: https://dx.doi.org/10.3748/wjg.v13.i10.1547