Age-related histomorphologic changes in the canine Gastrointestinal tract: A histologic and immunohistologic study

Table 1 Distribution of age, gender and breed

Age (yr)	Gender	Breed
0.25	M	Collie
0.5	M	Boxer
0.75	F	Rottweiler
0.75	M	West Highland White Terrier
2	F	Newfoundland
3	M	American Canadian White Shepard
3	M	Doberman-Pinscher
5	M	Fox Terrier
5	M	Mongrel
6	M	Pommeranian
6	F	German Shepherd Dog
7	M	Bernese Mountain Dog
7	F	Schnauzer
7	F	Caucasian Shepherd
7	F	Rottweiler
8	M	Mongrel
8	M	German Shorthaired Pointer
8.5	F	Mongrel
9	F	Mongrel
10	M	Mongrel
12	F	English Setter
12	M	Mongrel
12	M	Gordon Setter
13	F	Hovawart
13	F	Mongrel
14	F	Mongrel
14	F	Rottweiler
17	F	Mongrel

M: Male; F: Female.

Table 2 Absolute values in the stomach

Age(yr)	Depth offoveolae(μm)	Glandularlength(μm)	Chiefcells(%)	Fibrelayers(n)	Muscularismucosa(μm)	Submucosa(μm)	Tunicamuscularis(μm)
0.5	210	952	40	6	89.8	60	2076
0.75	226	1073	40	3	99.3	1157	2341
0.75	146	1076	60	4	146.8	365	1746
2	152	1249	70	6	133.0	866	2526
3	155	795	50	11	96.8
3	228	1052	0	4	212.5	341	1600
5	169	942	70	4	142.3	867	2221
5	198	1013	60	5	96.5	972	1652
7	139	1012	40	3	140.0	869	3140
8	204	927	70	2	192.8	524	1488
8	141	774	30	7	136.8	653	2684
8	219	1074	30	4	154.8	169	968
9	167	986	60	4	249.0	551	2974
10	220	1146	40	6	122.8	1075	2337
12	245	1017	50	9	178.8	3501	2875
12	166	1165	40	5	112.8
12	230	1059	70	6	121.3	1102	2191
14	189	795	20	12	154.5	1250	1205
17	294	815	20	6	120.5	694	2038

Table 3 Absolute values in the jejunum (μm)

Age(yr)	Villuslength	Cryptdepth	Stratumcompactum	Muscularismucosa	Submucosa	Circulartunicamuscularis	Longitudinaltunicamuscularis
0.25	572	448	17	58	92	443	254
0.5	622	354	30.75	52	327	573	387
0.75	981	1051	24.25	82	273	729	478
0.75	570	269	35	56	193	519	304
2	536	435	39.75	88	161	542	182
5	894	412	67.25	67	229	764	368
5	882	303	37.75	62	183	658	338
6	867	406	62.75	68	217	585	249
7	589	623	45	73	176	602	346
7	906	466	32.25	99	228	766	454
7	630	381	56.25	106	292	639	418
8	596	333	87.25	162	232	802	285
12	647	314	108.25	150	294	1152	446
12	653	426	46.75	59	161	579	334
13	703	506	73	112	364	1047	217
13	709	344	53.4	146	339	672	290
14	726	475	44.75	89	267	793	370
14	700	514	104.2	136	233	971	535
17	695	403	40.5	75	309	693	304

Table 4 Absolute values in the colon (μm)

Age(yr)	Cryptlength	Muscularismucosa	Submucosa	Circulartunicamuscularis	Longitudinaltunicamuscularis
0.25	520	41.3	122	387	1235
0.5	530	40.3	165	454	707
0.75	488	60.0	203	338	537
0.75	515	52.8	133	540	530
2	515	56.3	362	541	562
5	609	54.0	388	557	968
5	577	82.3	349	792	682
6	533	48.3	531	728	701
6	363	51.0	375	449	855
7	716	77.7	299	617	1307
7	443	63.0	165	534	586
7	404	38.1	146	519	553
8.5	343	73.0	162	812	787
10	567	44.5	223	734	1034
12	485	40.5	462	510	568
12	460	59.5	372	641	810
13	399	45.5	191	776	1780
13	612	72.5	201	810	981
14	481	37.8	1397	784	575
17	607	48	315	607	359

Table 5 Proliferation in the jejunum

Age (yr)	Proliferative length epithelium		Proliferating cells (n)
	Absolute(μm)	Relative(%)	Villous laminapropria (/villus)	Intercrypt lamina propria(/intercrypt space)	Lamina propriabelow crypts	Muscularis mucosa(/mm length)	Submucosa(/mm length)	Tunica muscularis(/mm length)
0.25	296.4	69.3	4.57	0.77	22.62	8.25	6.25	1.28
0.5	179.2	75.2	25.21	2.16	35.50	10.50	1.34	0.37
0.75	278.4	61.5	3.79	1.22	14.39	5.19	2.99	0.58
0.75	1006.4	77.0	1.23	0.43	31.07	0.00	0.00	0.06
2	151.2	47.6	1.11	0.29	2.89	0.00	0.00	0.00
5	277.6	70.3	7.48	1.16	5.56	4.17	1.64	0.33
5	314.4	73.1	9.53	0.68	17.84	1.66	1.14	0.21
6	243.8	76.8	3.24	0.00	1.06	0.98	0.00	0.24
7	378.6	71.6	2.25	0.24	8.82	0.96	0.00	0.00
7	388.0	73.2	1.61	0.47	4.66	1.49	0.18	0.00
7	412.4	70.3	7.81	0.00	4.04	0.00	1.04	0.20
8	219.6	57.7	2.89	0.00	2.86	0.39	0.00	0.00
12	139.0	60.8	4.43	0.31	13.20	0.00	0.00	0.00
12	358.0	77.6	6.78	1.33	15.14	0.00	1.65	0.20
13	231.6	77.4	16.18	1.03	19.14	0.91	0.26	0.93
13	291.4	66.8	1.38	0.00	0.00	0.00	0.00	0.00
14	335.2	73.0	2.42	0.29	1.67	0.64	1.12	0.31
14	369.4	77.1	15.55	0.75	15.04	2.70	0.18	0.09
17	248.0	65.8	0.81	0.23	2.65	0.00	0.00	0.00

Table 6 Proliferation in the colon

Age (yr)	Proliferative length epithelium		Proliferating cells (n)
	Absolute(μm)	Relative(%)	Intercrypt lamina propria(/intercrypt space)	Lamina propriabelow crypts	Muscularis mucosa(/mm length)	Submucosa(/mm length)	Tunica muscularis(/mm length)
0.25	147.4	28.8	2.117	1.59	32.07	5.21	1.33
0.5	179.0	32.2	0.943	1.67	9.20	2.63	0.12
0.75	90.2	20.3	0.388	0.36	0.98	0.78	0.04
0.75	217.8	39.4	2.048	0.79	1.64	1.21	0.09
2	129.8	27.8	0.194	0.28	0.00	0.39	0.00
5	192.0	32.9	2.251	0.89	3.02	1.14	0.17
5	219.5	36.9	0.657	0.71	2.63	0.37	0.11
6	189.2	56.4	0.276	0.11	1.05	0.43	0.59
6	194.4	37.4	0.563	0.33	1.35	0.62	0.10
7	118.4	29.8	0.248	0.00	0.00	0.40	0.00
7	158.0	31.8	0.226	0.18	0.00	1.12	0.05
7	215.8	33.3	0.838	0.63	2.01	0.52	0.00
8.5	150.0	37.1	0.583	0.24	1.12	0.00	0.05
10	132.8	26.1	1.059	1.60	3.18	0.21	0.04
12	151.8	32.8	1.031	1.49	6.13	0.27	0.16
12	173.4	33.9	0.6519	0.55	1.84	0.29	0.00
13	132.8	26.1	0.000	0.33	1.79	0.85	0.20
13	163.6	25.5	0.817	0.29	0.66	0.24	0.02
14	138.2	30.9	0.831	0.37	3.27	0.00	0.00
17	234.0	39.4	0.329	0.40	0.00	0.18	0.00

Table 7 Strength of correlation between age and diameter of different layers in the stomach wall of dogs

	All ages	< 10 yr
Absolute foveolar depth	0.44	0.05
Relative foveolar depth	0.54	0.11
Lamina muscularis mucosa	0.17	0.58
Submucosa	0.35	0.17
Tunica muscularis	-0.04	0.01

Table 8 Strength of correlation between age and diameter of different layers in the jejunal wall of dogs

	All ages	< 10 yr
Villus length	-0.01	0.17
Crypt depth	-0.15	-0.2
Whole mucosa	0.5	0.1
Lamina propria	0.54	0.72
Lamina muscularis mucosa	0.51	0.61
Submucosa	0.46	0.13
Circular muscle layer	0.59	0.6
Longitudinal muscle layer	0.12	0.12

Table 9 Strength of correlation between age and diameter of different layers in the colonic wall of dogs

	All ages	< 10 yr
Crypt depth	0.01	-0.21
Lamina muscularis mucosa	-0.05	0.42
Submucosa	0.38	0.27
Circular muscle layer	0.61	0.63
Longitudinal muscle layer	0.2	0.17

Table 10 Strength of correlation between age and number of proliferating cells in the different wall layers of the jejunum and colon

	Jejunum		Colon
	All ages	< 10 yr	All ages	< 10 yr
Absolute length of proliferating epithelium	-0.21	-0.16	0.07	0.17
Relative length of proliferating epithelium	0.15	0.07	0.02	0.37
Lamina propria of villi	-0.07	-0.28
Lamina propria between crypts	-0.32	-0.62	-0.36	-0.43
Lamina propria below crypts	-0.46	-0.74	-0.24	-0.65
Lamina muscularis mucosa	-0.56	-0.61	-0.36	-0.5
Submucosa	-0.45	-0.54	-0.57	-0.61
Tunica muscularis	-0.29	-0.56	-0.36	-0.34