Neutrophil depletion-but not prevention of Kupffer cell activation-decreases the severity of cerulein-induced acute pancreatitis

Table 1 Pancreatic injury in control (CONT) mice and mice treated with cerulein (CER)

	CONT	CER
Serum amylase (UI/L)	1582 [1236 - 1850]	19528 [16369 - 26457]	P < 0.0001
Pancreatic water content (% wet weight)	0.74 [0.71 – 0.76]	0.80 [0.78 - 0.83]	P < 0.0001
Pancreatic MPO activity (U/mg tissue dry weight)	322 [125 - 456]	2738 [1978 - 3245]	P = 0.0002
Pancreatic necrosis (%)	1.26 [0.5 – 2.3]	27.7 [24.5 – 35.0]	P = 0.0001

MPO = myeloperoxidase. n ≥ 5 in each group. Data in CER and CONT groups were compared with Mann-Whitney test.

Table 2 Pulmonary injury in control (CONT) mice and mice treated with cerulein (CER)

	CONT	CER
Pancreatic water content (% wet weight) 0.74 [0.71 – 0.76] 0.80 [0.78 - 0.83] P < 0.0001 Pancreatic MPO activity (U/mg tissue dry weight) 322 [125 - 456] 2738 [1978 - 3245] P = 0.0002 Pancreatic necrosis (%) 1.26 [0.5 – 2.3] 27.7 [24.5 – 35.0] P = 0.0001 CONT CER Pulmonary MPO activity (U/mg tissue dry weight) 947 [782 - 1145] 10173 [8710 - 12035] P < 0.0001 Pulmonary permeability index 0.57 [0.5 – 0.8] 3.9 [3.1 - 5.1] P = 0.0002 Pulmonary MPO activity (U/mg tissue dry weight)	947 [782 - 1145]	10173 [8710 - 12035]	P < 0.0001
Pulmonary permeability index	0.57 [0.5 – 0.8]	3.9 [3.1 - 5.1]	P = 0.0002

MPO = myeloperoxidase. Pulmonary permeability index: ratio of FITC-labeled bovine albumin between bronchoalveolar lavage and serum. n ≥ 5 in each group. Pulmonary MPO activity and pulmonary permeability index were compared by Mann-Whitney test.

Table 3 IL-6 and IL-10 concentrations in serum, pancreas, lungs, and livers in control (CONT) mice and mice treated with cerulein (CER)

	CONT	CER
Serum IL-6 (pg/mL)	8.9 [8.7 - 11.4]	267.5 [98.0 - 312.0]	P = 0.02
Serum Il-10 (pg/mL)	11.8 [8.9 - 14.7]	18.7 [10.9 - 21.6]	P = 0.23
Pancreatic IL-6 (pg/mg protein)	1.2 [0.5 - 1.9]	8.4 [6.4 - 13.2]	P = 0.02
Pancreatic IL-10 (pg/mg protein)	0.08 [0.03 - 0.20]	0.32 [0.20 - 0.79]	P = 0.03
Pulmonary IL-6 (pg/mg protein)	1.5 [0.5 - 2.1]	3.0 [2.4 - 6.9]	P = 0.004
Pulmonary IL-10 (pg/mg protein)	0.10 [0.01 - 0.20]	0.87 [0.42 - 3.80]	P = 0.06
Hepatic IL-6 (pg/mg protein)	1.15 [0.80 - 1.60]	8.20 [5.60 - 11.52]	P = 0.006
Hepatic IL-10 (pg/mg protein)	0.12 [0.05 - 0.20]	0.43 [0.33 - 0.48]	P = 0.06

n ≥ 5 in each group. Data in CER and CONT groups were compared by Mann-Whitney test.