Pastor CM, Vonlaufen A, Georgi F, Hadengue A, Morel P, Frossard JL. Neutrophil depletion-but not prevention of Kupffer cell activation-decreases the severity of cerulein-induced acute pancreatitis. World J Gastroenterol 2006; 12(8): 1219-1224 [PMID: 16534874 DOI: 10.3748/wjg.v12.i8.1219]
Corresponding Author of This Article
Dr Catherine M Pastor, Laboratoire de Physiopathologie Hépatique et Imagerie Moléculaire, Hôpitaux Universitaires de Genève, Bâtiment C, Room 6-795, 24 Rue Micheli-du-Crest, 1205 Geneva, Switzerland. catherine.pastor@hcuge.ch
Article-Type of This Article
Basic Research
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World J Gastroenterol. Feb 28, 2006; 12(8): 1219-1224 Published online Feb 28, 2006. doi: 10.3748/wjg.v12.i8.1219
Neutrophil depletion-but not prevention of Kupffer cell activation-decreases the severity of cerulein-induced acute pancreatitis
Catherine M Pastor, Alain Vonlaufen, Fabianna Georgi, Antoine Hadengue, Philippe Morel, Jean-Louis Frossard
Catherine M Pastor, Laboratoire de Physiopathologie Hépatique et Imagerie Moléculaire, Hôpitaux Universitaires de Genève, Bâtiment C, Room 6-795, 24 Rue Micheli-du-Crest, 1205 Geneva, Switzerland
Alain Vonlaufen, Division of Gastroentérologie et Hépatologie, Hôpitaux Universitaires de Genève, 24 Rue Micheli-du-Crest, 1205 Geneva, Switzerland
Fabianna Georgi, Département APSI, Hôpitaux Universitaires de Genève, 24 Rue Micheli-du-Crest, 1205 Geneva, Switzerland
Antoine Hadengue, Division of Gastroentérologie et Hépatologie, Hôpitaux Universitaires de Genève, 24 Rue Micheli-du-Crest, 1205 Geneva, Switzerland
Philippe Morel, Département de Chirurgie, Hôpitaux Universitaires de Genève, 24 Rue Micheli-du-Crest, 1205 Geneva, Switzerland
Jean-Louis Frossard, Division of Gastroentérologie et Hépatologie, Hôpitaux Universitaires de Genève, 24 Rue Micheli-du-Crest, 1205 Geneva, Switzerland
Author contributions: All authors contributed equally to the work.
Supported by a grant from the Fonds National Suisse de la Recherche Scientifique N° 3200B0-100764 to Jean-Louis Frossard
Correspondence to: Dr Catherine M Pastor, Laboratoire de Physiopathologie Hépatique et Imagerie Moléculaire, Hôpitaux Universitaires de Genève, Bâtiment C, Room 6-795, 24 Rue Micheli-du-Crest, 1205 Geneva, Switzerland. catherine.pastor@hcuge.ch
Telephone: +41-22-3729353 Fax: +41-22-3729366
Received: June 30, 2005 Revised: July 7, 2005 Accepted: July 15, 2005 Published online: February 28, 2006
Abstract
AIM: To determine whether neutrophil depletion and Kupffer cell inhibition might combine their protective effects to decrease the severity of acute pancreatitis.
METHODS: Mice had cerulein administration to induce acute pancreatitis and were pretreated with either anti-mouse neutrophil serum or gadolinium chloride (GdCl3) to prevent Kupffer cell activation, or both treatments. Injury was assessed in pancreas and lungs. Myeloperoxidases (MPO) assessed neutrophil infiltration. Interleukin-6 (IL-6) and IL-10 were measured in serum, pancreas, lungs and liver.
RESULTS: In mice with acute pancreatitis, neutrophil depletion reduced the severity of pancreatitis and pancreatitis-associated lung injury. Kupffer cell inactivation by GdCl3 had less protective effect, although IL-6 and IL-10 concentrations were significantly decreased. The protective treatment brought by neutrophil depletion was not enhanced by Kupffer cell inactivation and both treatments did not combine their protective effects.
CONCLUSION: Our results confirm the role of activated neutrophils in aggravating organ injury in acute pancreatitis while the role of Kupffer cell activation is less obvious.
