Association of H pylori cagA and vacA genotypes and IL-8 gene polymorphisms with clinical outcome of infection in Iranian patients with gastrointestinal diseases

Table 1 Primer sequences used for detection of cagA gene status and IL-8 or vacA gene polymorphisms

Locus	Primers	Size (bp)
IL-8 -251	Common primer, 5’-tgc ccc ttc act ctg tta ac-3’	336
	A allele, 5’-cca caa ttt ggt gaa tta tca at-3’
	T allele, 5’-cca caa ttt ggt gaa tta tca aa-3’
β-globin	5’- aca caa ctg tgt tca cta gc -3’	100
	5’- caa ctt cat cca cgt tca cc -3’
cagA	5’-aat aca cca acg cct cca ag-3’	400
	5’-ttg ttg ccg ctt ttg ctc tc-3’
vacA
S1 and s2	5’-ctg ctt gaa tgc gcc aaa c-3’	s1 = 259
	5’-atg gaa ata caa caa aca cac-3’	s2 = 286
m1 and m2	5’-gcg tct aaa taa ttc caa gg-3’	m1 = 570
	5’-caa tct gtc caa tca agc gag-3’	m2 = 645
glmM	5’-aag ctt tta ggg gtg tta ggg gtt t-3’	294
	5’-aag ctt act ttc taa cac taa cgc-3’

Table 2 IL-8 -251 A/T polymorphism, cagA status and vacA gene polymorphism in patients with gastric diseases

Locus	Genotype	Patients Gastritisn(%)	Peptic Ulcern(%)	Gastric Cancern(%)	Pvalue
IL-8 -251	AA	22 (14.4)	14 (23.0)	9.0 (47.4)	0.013
	AT	74 (48.4)	28 (45.9)	6.0 (31.6)
	TT	57 (37.3)	19 (31.1)	4.0 (21.1)
cagA	+	148 (74.4)	54 (80.6)	17 (85.0)	0.39
	-	51 (25.6)	13 (19.4)	3.0 (15.0)
vacA	s1m1	48 (26.5)	21 (32.3)	12.0 (66.7)	0.001
	s1m2	74 (40.9)	33 (50.8)	3.0 (16.7)
	s2m2	59 (32.6)	11 (16.9)	3.0 (16.7)