Kamali-Sarvestani E, Bazargani A, Masoudian M, Lankarani K, Taghavi AR, Saberifiroozi M. Association of H pylori cagA and vacA genotypes and IL-8 gene polymorphisms with clinical outcome of infection in Iranian patients with gastrointestinal diseases. World J Gastroenterol 2006; 12(32): 5205-5210 [PMID: 16937534 DOI: 10.3748/wjg.v12.i32.5205]
Corresponding Author of This Article
Eskandar Kamali-Sarvestani, Associate Professor of immunology, Department of Immunology, Medical School, Shiraz University of Medical Sciences, Shiraz, PO Box 71345-1798, Iran. immunol2@sums.ac.ir
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Rapid Communication
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World J Gastroenterol. Aug 28, 2006; 12(32): 5205-5210 Published online Aug 28, 2006. doi: 10.3748/wjg.v12.i32.5205
Association of H pylori cagA and vacA genotypes and IL-8 gene polymorphisms with clinical outcome of infection in Iranian patients with gastrointestinal diseases
Eskandar Kamali-Sarvestani, Department of Immunology and Autoimmune Diseases Research Center, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
Abdulah Bazargani, Department of Bacteriology and virology, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
Malihe Masoudian, Department of cellular and molecular sciences, Khatam University, Tehran, Iran
Ali-Reza Taghavi, Department of Internal medicine, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
Kamran Lankarani, Mehdi Saberifiroozi, Gastrointestinal and Hepatology Research center, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
Author contributions: All authors contributed equally to the work.
Supported by a grant numbered 82-1774 from Shiraz University of Medical Sciences
Correspondence to: Eskandar Kamali-Sarvestani, Associate Professor of immunology, Department of Immunology, Medical School, Shiraz University of Medical Sciences, Shiraz, PO Box 71345-1798, Iran. immunol2@sums.ac.ir
Telephone: +98-711-2304069
Received: April 7, 2006 Revised: May 20, 2006 Accepted: May 25, 2006 Published online: August 28, 2006
Abstract
AIM: To find out if a functional promoter polymorphism in the IL-8 gene along with cagA status and polymorphisms in vacA gene influence the type of diseases in Iranian patients infected by H pylori.
METHODS: IL-8 -251 A/T polymorphism was genotyped by oligonucleotide allele specific PCR (ASO-PCR) in a sample of 233 patients with H pylori infection undergoing upper gastrointestinal endoscopy. The presence of cagA gene and polymorphisms in vacA gene was also determined by PCR. Association of these genetic polymorphisms with the development of gastritis, peptic ulcers as well as gastric cancer was tested.
RESULTS: When the patients with different clinical manifestations were compared according to the presence of cagA gene or various vacA genotypes, only the vacA genotypes were significantly different among gastritis, peptic ulcer and gastric cancer patients (χ2 = 17.8; P = 0.001). Furthermore, there was a significant difference in the frequency of IL-8 -251 A/T genotypes between patients with gastric cancer and benign diseases (χ2 = 10.47; P = 0.005).
CONCLUSION: The IL-8 -251 A/T polymorphism and the polymorphisms in H pylori vacA gene are involved in limiting the infection outcome to gastritis and peptic ulcer or in favoring cancer onset in Iranian patients.
