Reversing multidrug resistance by RNA interference through the suppression of MDR1 gene in human hepatoma cells

Table 1 Sequences of shRNA oligonucloetides

Oligonu-cloetides	Sense + loop + antisense DNA template
MDR-A	5′-GATCCGCTTTGGCTGCCATCATCCAttcaagacgTGGATGATGG CAGCCAAAGTTTTTTGTCGACA-3′;
	3′-GCGAAACCGACGGTAGTAGGTaagttctgcACCTACTACCGT CGGTTTCAAAAAACAGCTGTTCGA-5′
MDR-B	5′-GATCCGGCCTAATGCCGAACACATttcaagacgATGTGTTCGGCATTAGG CCTTTTTTGTCGACA-3′; 3′-GCCGGATTACGGCTTGTGTAaagttctgcTACACAAGCCGTAATCCGGAA AAAACAGCTGTTCGA-5′

The underlined parts are loops of shRNA oligonucleotides.

Table 2 Effect of stable expression of shRNAs-mediated drug resistance to adriamycin

Cell line	Adriamycin IC50 (mg/L)	Resistance index (RI)
HepG2	0.141 ± 0.008	1
HepG2/ADM	14.394 ± 0.263a	102.085
Con	13.275 ± 0.353a	94.149
sh1	0.269 ± 0.035	1.908
sh 2	0.467 ± 0.617a	3.312

^aP < 0.05 vs ID50 value of HepG2. The data are presented as mean  ±  SD. Sh1, sh2 and con represent HepG2/ADM/pGenSil-l/MDR-B clone 1, clone 2, and the control HepG2/ADM/vec clone, respectively.