Viral Hepatitis
Copyright ©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. Nov 7, 2005; 11(41): 6416-6421
Published online Nov 7, 2005. doi: 10.3748/wjg.v11.i41.6416
Table 1 Oligonucleotide primers and PCR cycling profiles used in the study
FragmentPrimerPosition1SequenceDenaturationAnnealingExtensionSize2
A455(+)455-4745’-CAAGGTATGTTGCCCGTTTG-31 345
1 800(–)1 800-1 7735’-AGACCAATTTATGCCTACAGCCTCCTA-3’94 °C 30 s62 °C 30 s72 °C 90 s
B1 687(+)1 687-1 7085’-CGACCGACCTTGAGGCATAC-3’
685(-)704-6855’-CGAACCACTGAACAAATGGC-3’94 °C 30 s63 °C 30 s72 °C 30 S2 198
Table 2 Summary of cloning and sequencing results
Patient detailsPCR fragment1Number of clones
Chromosome number and length
No.SexAgeSequencedWith vectorWith HBVWithHBV/ chromosomalWith chromosomal
(yr2)DNADNA onlyDNADNA only
355F18B513017 (117 bp)
8225M23A302011 (278 bp)
B40400
28M26A402024 (512 bp)
16 (140 bp)
B40400
4038F27A605011 (97 bp)
B40400
52833F32A1226044 (456 bp)
10 (235 bp)
10 (330 bp)
2 (422 bp)
B40400
962M27A804131 (300 bp)
14 (410 bp)
17 (410 bp)
B50500