Polymorphism of CYPIA1 and GSTM1 genes associated with susceptibility of gastric cancer in Shandong Province of China

Table 1 Distribution of cases and controls by age, sex, education, and occupation

Variable	Value level	Gastric group		Control group		χ2	P
		n	%	n	%
Age (yr)	<35	4	3.9	18	29
	35-	13	12.7	21	33.9
	45-	85	83.3	23	37.1	38.945	<0.001
Sex	Male	86	84.3	33	53.2
	Female	16	15.7	29	46.8	18.718	<0.001
Education years	<5	57	55.9	14	22.6
	6-	29	28.4	28	45.2
	13-	16	15.7	20	32.3	17.807	<0.001
Occupation	Farmer	63	61.8	24	38.7
	Worker	21	20.6	18	29
	Card	18	17.6	20	32.3	8.573	0.014

Table 2 Relative risk of stomach cancer in relation to observed factors

Factors	Controls	Cases	OR1	95%CI		OR2	95%CI
Chinese sauerkraut
None	53	95	1
Sometime	6	4	0.37	0.08	1.58	0.24	0.04	0.95
Often (2+/wk)	3	3	0.56	0.09	3.62	0.39	0.41	3.3
Mildew food
None	59	94	1
Sometime	2	5	1.57	0.2	12.11	1.5	0.22	10.14
Often (2+/mo)	1	3	1.88	0.17	48.13	1	0.07	14.69
Eat raw garlic/yr
<1 000	20	77	1
>1 000	42	25	0.15	0.07	0.33	0.15	0.06	0.33
CYPIA1 genotype
A/A	35	53	1
A/G	24	27	0.74	0.35	1.58	0.59	0.26	1.34
G/G	3	22	4.84	1.24	22.07	5.91	1.28	27.24
H pyloriinfection
-	26	13	1
+	36	89	4.64	2.15	11.51	4	1.65	9.71
Drinking white alcohol3
None	37	48	1
1-00	8	9	0.87	0.27	2.77	1.03	0.26	4.02
100+	17	45	2.04	0.95	4.39	1.11	0.41	2.99
Smoking4
None	42	46	1
<270	14	15	0.98	0.39	2.46	0.66	0.22	1.94
≥270	6	41	6.23	2.24	18.27	2.78	0.9	8.65
History of cancer family
None	59	88	1
Yes	3	14	3.13	0.79	14.38	2.98	0.69	12.82
GSTM1 genotype
+/+ or +/O	36	33	1
O/O	26	67	2.81	1.39	5.71	2.72	1.32	5.62

¹Non-adjusted OR,

²adjusted for age, sex, education, and occupation,

³drinking index = white alcohol/month×number of drinking years^,4smoking index = cigarette/day × number of smoking years.

Table 3 Relative risk of stomach cancer for combined CYPIA1 and GSTM1 genotypes

CYPIA1	GSTM1	Controls	Cases	OR1	95%CI
A/A	+/+ or+/O	22	17	1.00
	O/O	13	35	3.34	1.13	9.87
A/G	+/+ or+/O	13	10	0.65	0.20	2.14
	O/O	11	16	1.67	0.52	5.36
G/G	+/+ or+/O	1	6	4.16	0.40	43.45
	O/O	2	16	16.48	2.36	115.0

¹Adjusted for age, sex, education, and occupation; CI: confidence interval

Table 4 Relative risk of stomach cancer for combined CYPIA1 genotype and H pylori infectionl

CYPIA1	H pylori	Controls	Cases	OR1	95%CI
A/A	-	18	5	1.00
	+	17	22	7.19	1.98	26.11
A/G	-	7	5	1.59	0.29	8.91
	+	17	48	2.94	0.78	11.13
G/G	-	1	3	13.18	0.42	414.9
	+	2	19	28.11	3.95	199.8

¹Adjusted for age, sex, education, and occupation; CI: confidence interval.

Table 5 Relative risk of stomach cancer for combined CYPIA1 genotype and smoking

CYPIA1	Smoking	Controls	Cases	OR1	95%CI
A/A	None	21	21	1.00
	Yes	14	32	1.53	0.47	4.96
A/G	None	18	12	0.62	0.20	1.89
	Yes	6	15	0.92	0.24	3.53
G/G	None	3	13	4.90	0.90	26.73
	Yes	0	9	19.02	7.27	54.06

¹Adjusted for age, sex, education, and occupation; CI: confidence interval; ²weighed OR by adding to 0.5 for each cell number.

Table 6 Relative risk of stomach cancer for GSTM1 genotype and H pylori infection

GSTM1	H p	Controls	Cases1	OR2	95%CI
+/+ or +/O	-	16	5	1.00
	+	20	28	4.19	1.16	15.13
O/O	-	10	7	3.53	0.70	17.81
	+	16	60	12.37	3.37	45.46

¹Adjusted for age, sex, education, and occupation; CI: confidence interval;

²two cases missing.

Table 7 Relative risk of stomach cancer for combined GSTM1 genotype and smoking

GSTM1	Smoking	Controls	Cases1	OR2	95%CI
+/+ or +/O	-	23	16	1
	+	13	17	1.22	0.37	4.01
O/O	-	19	30	3.08	1.11	8.54
	+	7	37	4.32	1.27	14.69

¹Adjusted for age, sex, education, and occupation; CI: confidence interval;

²two cases missing.