Changes of the intestinal endocrine cells in the C57BL/6 mouse after implantation of murine lung carcinoma (3LL): An immunohistochemical quantitative study

doi:10.3748/wjg.v11.i35.5460

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Sep 21, 2005; 11(35): 5460-5467
Published online Sep 21, 2005. doi: 10.3748/wjg.v11.i35.5460

Table 1 Antisera used in this study

Antisera raised¹	Code	Source	Dilution
CGA²	A430	DAKO Corp., Carpinteria, CA, USA	1:1 000
Serotonin	BO68082C	BioGenex Lab., San Ramon, CA, USA	1:20
Somatostatin	PUO421295	BioGenex Lab., San Ramon, CA, USA	1:20
Glucagon	927604	Dia Sorin, Stillwater, MN, USA	1:2 000
Gastrin	PUO190796	BioGenex Lab., San Ramon, CA, USA	1:20
CCK-8²	750257	Dia Sorin, Stillwater, MN, USA	1:500
HPP2	A610	DAKO Corp., Carpinteria, CA, USA	1:600

¹All antisera were raised in rabbits.

²CGA: chromogranin A, CCK-8: cholecystokinin-8, hPP: human pancreatic polypeptide.

Table 2 Regional distributions and frequencies of the endocrine cells in the small intestinal tract of the C57BL/6 mouse after implantation of 3LL

Antisera	Non-implanted Sham			3LL-implanted group
	Duodenum	Jejunum	Ileum	Duodenum	Jejunum	Ileum
CGA¹	24.70±4.11	21.10±3.70	9.30±1.95	13.20±3.79^b	7.20±3.12^b	2.10±1.29b
Serotonin	22.80±4.76	12.20±4.24	6.10±1.73	11.10±4.20^b	9.10±1.60^a	4.10±0.99b
Somatostatin	1.70±3.01	6.10±1.91	2.60±0.84	1.60±0.84	0.90±0.57^b	1.40±0.70b
Glucagon	ND²	ND	1.40±0.52	ND	ND	ND
CCK-81	8.30±2.71	3.40±1.35	ND	9.40±2.55	1.30±0.48b	ND

mean±SD, number of IR cells among 1 000 epithelial and intestinal acinar cells (1 field in each animal, total 10 fields);

¹CGA: chromogranin A, CCK-8: cholecystokinin-8;

²ND: not detected;

^aP<0.05 vs non-implanted Sham by M–W test;

^bP<0.01 vs non-implanted Sham by M–W test.

Table 3 Regional distributions and frequencies of the endocrine cells in the large intestinal tract of the C57BL/6 mouse after implantation of 3LL

Antisera	Non-implanted Sham			3LL-implanted group
	Cecum	Colon	Rectum	Cecum	Colon	Rectum
CGA¹	7.30±2.45	14.70±4.85	3.20±1.14	2.20±1.14b	8.80±3.19^b	0.80±0.63b
Serotonin	12.30±3.97	29.40±9.41	37.80±9.75	7.10±1.37^b	11.80±3.74^b	1.80±1.03b
Somatostatin	ND²	ND	ND	ND	ND	ND
Glucagon	ND	0.60±0.52	ND	ND	ND	ND
CCK-81	ND	ND	ND	ND	ND	ND

mean±SD, number of IR cells among 1 000 epithelial and intestinal acinar cells (1 field in each animal, total 10 fields);

¹CGA: chromogranin A, CCK-8: cholecystokinin-8;

²ND: not detected;

^bP<0.01 vs non-implanted Sham by M–W test.

Citation: Ku SK, Seong SK, Kim DY, Lee HS, Kim JD, Choi HY, Seo BI, Lee JH. Changes of the intestinal endocrine cells in the C57BL/6 mouse after implantation of murine lung carcinoma (3LL): An immunohistochemical quantitative study. World J Gastroenterol 2005; 11(35): 5460-5467
URL: https://www.wjgnet.com/1007-9327/full/v11/i35/5460.htm
DOI: https://dx.doi.org/10.3748/wjg.v11.i35.5460