Basic Research
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 21, 2005; 11(35): 5460-5467
Published online Sep 21, 2005. doi: 10.3748/wjg.v11.i35.5460
Changes of the intestinal endocrine cells in the C57BL/6 mouse after implantation of murine lung carcinoma (3LL): An immunohistochemical quantitative study
Sae-Kwang Ku, Seung-Kyoo Seong, Dae-Young Kim, Hyeung-Sik Lee, Jong-Dae Kim, Hae-Yun Choi, Bu-Il Seo, Jae-Hyun Lee
Sae-Kwang Ku, Seung-Kyoo Seong, Pharmacology and Toxicology Laboratory, Central Research Laboratories, Dong-Wha Pharmaceutical Industrial Co, Ltd, Anyang 430-017, Republic of Korea
Dae-Young Kim, Developmental Biology/Embryology, Division of Biological Science, Gachon Medical School, Kanghwha 417-840, Republic of Korea
Hyeung-Sik Lee, Department of Herbal Biotechnology, Daegu Haany University, Gyeongsan 712-715, Republic of Korea
Jong-Dae Kim, Hae-Yun Choi, Bu-Il Seo, College of Oriental Medicine, Daegu Haany University, Gyeongsan 712-715, Republic of Korea
Jae-Hyun Lee, Laboratory of Histology, College of Veterinary Medicine, Kyungpook National University, Taegu 702-701, Republic of Korea
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Hyeung-Sik Lee, Department of Herbal Biotechnology, Daegu Haany University, Gyeongsan 712-715, Republic of Korea. endohist@dhu.ac.kr
Telephone: +82-53-819-1436 Fax: +82-53-819-1574
Received: March 7, 2005
Revised: April 6, 2005
Accepted: April 9, 2005
Published online: September 21, 2005
Abstract

AIM: To study the distributions and frequencies of intestinal endocrine cells in the C57BL/6 mouse with immunohistochemical method using seven types of specific antisera against chromogranin A (CGA), serotonin, somatostatin, glucagons, gastrin, cholecystokinin (CCK)-8 and human pancreatic polypeptide (hPP) after abdominal subcutaneous implantation of murine lung carcinoma (3LL).

METHODS: The experimental animals were divided into two groups, one is non-implanted Sham and the other is 3LL-implanted group. Samples were collected from six regions of intestinal tract at 28th d after implantation of 3LL cells (1×105 cell/mouse).

RESULTS: In this study, five types of immunoreactive (IR) cells were identified except for gastrin and hPP. The regional distributions of the intestinal endocrine cells in the 3LL-implanted group were similar to those of the non-implanted Sham. However, significant decreases of IR cells were detected in 3LL-implanted group compared to those of non-implanted Sham. CGA- and serotonin-IR cells significantly decreased in 3LL-implanted groups compared to that of non-implanted Sham. Somatostatin-IR cells in the jejunum and ileum and CCK-8-IR cells in the jejunum of 3LL-implanted groups significantly decreased compared to that of non-implanted Sham. In addition, glucagon-IR cells were restricted to the ileum and colon of non-implanted Sham.

CONCLUSION: Implantation of tumor cell mass (3LL) induced severe quantifiable changes of intestinal endocrine cell density and the abnormality in density of intestinal endocrine cells may contribute to the development of gastrointestinal symptoms such as anorexia and indigestion, frequently encountered in patients with cancer.

Keywords: Intestinal endocrine cell; Immunohistochemistry; C57BL/6 mouse; 3LL; Tumor