Effect of NS398 on metastasis-associated gene expression in a human colon cancer cell line

Table 1 Up- and down-regulated genes in NS398-treated LoVo cells for 24 h (mean±SD)

Accession number	Gene descriptions	Ratio
Not found	Hs.23723, “ESTs” (346390)	2.67±1.66
Not found	Hs.106513, “EST” (348242)	2.31±0.67
BC040844	Hs.198253, NS1-associated protein 1 (435598)	1.96±0.25
BC011714	Hs.9605, heterogeneous nuclear ribonucleoprotein D-like (190165)	2.08±0.46
Not found	Hs.277401, ESTs (35363)	2.04±0.13
NT_024524	Hs.277704, PIBF1 gene product (124966)	2.29±0.81
BC007674	Hs.180414, CD24 antigen (small cell lung carcinoma cluster 4 antigen) (115306)	1.98±0.22
NM_003127	Hs.87497, spectrin, alpha, non-erythrocytic 1 (alpha-fodrin, 3021698)	2.27±0.53
Not found	Hs.272073, ESTs (1296662)	1.95±0.24
BT007404	Hs.8037, CD24 antigen (small cell lung carcinoma cluster 4 antigen, 124098)	0.49±0.04
K01500	Hs.18443, alpha-1-antichymotrypsin (117439)	0.51±0.05
Not found	Hs.45209, EST(2118886)	0.49±0.04
NM_005564	LCN2 (oncogene 24p3, 595821)	0.47±0.17
NM_001022	Hs.43913, ribosomal protein S19 (453963)	0.53±0.06
NM_003259	Intercellular adhesion molecule 5, telencephalin (ICAM5, 180864)	0.36±0.14
NM_002228	v-jun avian sarcoma virus 17 oncogene homolog (JUN, 823612)	0.51±0.07
NM_002908	v-rel avian reticuloendotheliosis viral oncogene homolog (REL, 2723459)	0.45±0.06

Table 2 (PDF) Up- and down-regulated genes in NS398-treated LoVo cells for 48 h (mean±SD)

Accession number	Gene description	Ratio
Not found	Hs.23723, “ESTs” (346390)	3.41±1.76
Not found	Hs.106513, “EST” (348242)	3.05±1.88
BC040844	Hs.198253, NS1-associated protein 1 (435598)	2.51±0.53
AF257505	Hs.23317, butyrophilin, subfamily 3, member A2 (219410)	2.12±0.56
NT_033899	Hs.28043, KIAA0712 gene product (219914)	2.13±0.40
BC011714	Hs.9605, heterogeneous nuclear ribonucleoprotein D-like (190165)	2.34±0.51
M62782	Hs.22907, human insulin-like growth factor binding protein 5 (IGFBP5) mRNA (31397)	2.15±0.43
NM_003127	Hs.87497, spectrin, alpha, non-erythrocytic 1 (alpha-fodrin, 3021698)	2.14±0.13
BC008005	Hs.116459, nucleotide binding protein 2 (E coli MinD like, 2498589)	2.01±0.29
Not found	Hs.43913, ESTs (2498857)	2.20±0.28
Not found	Hs.272073, ESTs (1296662)	2.33±0.43
BM508995	Hs.106513, ESTs, highly similar to proteasome (H sapiens, 1302647)	2.47±0.76
NM_000624	Hs.150580, alpha-1-antichymotrypsin (1322220)	2.14±0.35
AF248734	Hs.87497, apoptotic protease activating factor (963055)	2.28±0.45
BC002965	Lysosomal-associated membrane protein 2 (LAMP2), transcript variant LAMP2A (134418)	2.34±0.69
NM_006536	Chloride channel, calcium activated, family member 2 (CLCA2, 781187)	2.39±0.42
M90657	Tumor-associated antigen L6 (1964132)	2.85±1.58
Not found	Spliceosome associated protein 145 (1964680)	2.28±0.34
AW674474	Putative insulin-like growth factor ii associated (229316)	2.32±0.50
AF041835	Laminin, gamma 3 (LAMC3, 2497685)	2.16±0.48
AB019987	Chromosome-associated polypeptide-c (2597847)	2.06±0.09
AW277011	Putative vacuolar protein sorting-associated protein c (2744695)	2.20±0.64
BC032547	Homeo box A1 (HOXA1, 3923611)	2.27±1.18
NM_003391	Wingless-type MMTV integration site family member 2 (WNT2, 149373)	2.27±0.28
BC027948	c-fos induced growth factor (VEGF D, FIGF, 160946)	2.15±0.27
NM_001792	Cadherin 2, N-cadherin (neuronal, CDH2, 3617894)	2.03±0.10
NM_001964	Early growth response 1 (EGR1, 182411)	2.61±0.47
AF071400	Plasminogen activator inhibitor, type II (arginine-serpin, PAI2, 323255)	2.41±0.39
NM_002447	Macrophage stimulating 1 receptor (c-met-related tyrosine kinase, MST1R, 586698)	2.45±0.62
NM_003254	Tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity,
	collagenase inhibitor, TIMP1, 771755)	2.02±0.18
NM_003182	Tachykinin, precursor 1 (substance K, substance P, neurokinin 1, neurokinin 2, neuromedin L,	2.22±0.30
	neurokinin alpha, neuropeptide K, neuropeptide gamma, TAC1), transcript variant beta (784179)
BC004986	Hs.77202, ribosomal protein S25 (178052)	0.49±0.06
BC035128	Hs.23317, Max-interacting protein (130696)	0.45±0.05
Not found	Hs.23954, ESTs (132543)	0.51±0.06
AJ001810	Hs.106513, pre-mRNA cleavage factor Im (25 ku)	0.48±0.01
BC053521	66834, Hs.76847, spectrin, alpha, non-erythrocytic 1 (alpha-fodrin, 31230)	0.47±0.15
M64716	Hs.234726, ribosomal protein S25 (4932742)	0.46±0.05
BC032589	No, beta-2-microglobulin (1907327)	0.48±0.11
Not found	Hs.45209, EST (2118886)	0.47±0.11
NT_024524	Hs.15058, PIBF1 gene product (1596167)	0.50±0.05
NM_001779	CD58 antigen (lymphocyte function-associated antigen 3, CD58, 490368)	0.50±0.15
NM_002228	v-jun avian sarcoma virus 17 oncogene homolog (JUN, 823612)	0.41±0.11
NM_020979	Adaptor protein with pleckstrin homology and src homology 2 domains (APS, 3056093)	0.49±0.17
NM_000059	Breast cancer 2, early onset (BRCA2, 3850805)	0.30±0.10
NM_001223	Caspase 1, apoptosis-related cysteine protease (interleukin 1, beta, convertase, CASP1, 3858119)	0.48±0.09

The number in parenthesis represents the IMAGE clone number.