Mismatch repair genes (hMLH1, hPMS1, hPMS2, GTBP/hMSH6, hMSH2) in the pathogenesis of hepatocellular carcinoma

Table 1 Relation between reduced expression of the MMR genes and clinicopathological features of HCC patients.

Clincopathological features	No.	GTBP	hPMS1	hPMS2	hMLH1	hMSH2	HCV-RT-PCR	HBV-PCR
Age (yr) <50	9	6	0	5	5	7	6	0
>50	24	11	2	6	11	16	19	0
Gender M	18	8	2	4	9	13	12	0
F	15	9	0	7	7	10	13	0
Grade I and II	24	12	2	8	15	16	17	0
III	9	5	0	3	1	7	8	0
CirrhosisPresent	15	7	1	2	8	12	14	0
Absent	18	9	1	9	8	11	11	0
Total	33	17	2	11	16	23	25	0

Table 2 Oligonucleotide sequences of primers β-actin, MMR genes, HCV, and HBV.

Gene sequence	Size (bp)	Temperature (°C)
β-actin
5'-ACA CTG TGC CCA ACG AGG-3’	621	55-59
5'-AGG GGC CGG TCA T AC T-3’
hMPS-2
5'-TGC ATG CAG GAT TTG GAA A-3’	385	55
5'-GAA CCC CTC AGA ATC CAC GGA-3’
GTBP
5'-CCC TCA GCC ACC AAA GAA GCA-3’	288	56
5'-CTG CCA CCA CTT CCT CAT CCC-3’
hMLH-1
5'-GTG CTG GCA ATC AGG GAC CC-3’	215	58
5'-CAC GGT TGA GGC ATT GGG TAG-3’
hMPS-1
5'-GCG GCA ACA GTT CGA CTC CTT-3’	174	57
5'-AGC CTT GAT ACC CTC CCC GTT-3’
hMSH-2
5'-GTC GGC TTC GTG CGC TTC TTT-3’	429	58
5'-TCT CTG GCC ATC AAC TGC-3’ HCV
RB6A
5'-GTG AGG AAC TAC TGT CTT CAC G-3	266	55
RB6B
5'-ACT CGC AAG CAC CCT ATC AGG-3’
HBV
LBL
5'-CGG ATC CGT GGA GTT ACT CTG	460	55
GTT TTT GC-3’
RBL
5'-GCA AGC TCT AAC AAC AGT AGT TTC CCG G-3’

Table 3 Correlation confident analysis of the association between the five MMR gene expressions.

MMR	hMSH2	hPMS2	GTBP	hPMS1
hMLH1	13/29	6/30	9/30	1/30
	P = 0.0308a	P = 0.178	P = 0.39	P = 0.5
hPMS1	2/32	0/33	1/30
	P = 0.407	P = 0.391	P = 0.95
GTBP
	13/30	8/32
	P = 0.149	P = 0.0034a
hPMS2	6/32
	P = 0.407

^aP = 0.05 was considered statistically significant.