Mutations of p53 gene exons 4-8 in human esophageal cancer

Table 1 Characteristics of patients with squamous cell esophageal cancer.

Patient No.	Age(yr)	Sex	Location of tumor	Therapy of preoperation	Time of operation	Sample
						Tumor	Adjacent tissue
1	65	M	Upper		1997	+	-
2	48	M	Upper	Radiotherapy	1997	+	+
3	39	M	Lower		1998	+	-
4	61	F	Lower		1998	+	+
5	40	M	Lower		1998	+	+
6	70	F	Mid	Radiotherapy	1998	-	+
7	54	M	Mid		1998	-	+
8	70	F	Lower		1999	+	+
9	50	M	Mid		1999	+	+
10	66	M	Upper		1999	+	+
11	43	M	Mid		1999	+	+
12	60	M	Upper	Radiotherapy	1999	+	+
13	62	M	Upper		1999	+	+
14	60	F	Upper		1999	+	+
15	51	M	Upper		1999	+	+
16	67	M	Upper		1999	+	+
17	53	F	Mid		1999	+	+
18	49	M	Upper		1999	+	-
19	57	F	Lower		1999	+	-
20	61	M	Lower	Chemotherapy	1999	+	+
21	63	M	Lower		1999	+	-
22	65	F	Mid	Radiotherapy	1999	+	-
23	60	M	Mid		1999	+	+
24	54	M	-		1999	+	-

Upper: upper thoracic esophageal cancer, Mid: mid-thoracic esophageal cancer, Lower: lower thoracic esophageal cancer.

Table 2 p53 gene mutations in tumor tissue from esophageal cancer patients.

No.	Exon	Codon	Nucleotide	Amino acid
1	None
2	4	111	CTG-CTA	Leu-Leu
	4	131	GGG-GAG	Gly-Glu
3	None
4	5	179	CCA-CTA	Pro-Leu
5	None
6	-	-	-	-
7	-	-	-	-
8	4	107	TAC-TAT	Tyr-Tyr
9	None
10	None
11	4	102	ACC-ACT	Thr-Thr
	4	135	CAT-TAT	His-Tyr
	5		16 bp deletion
12	None
13	None
14	4	131	GGG-GAG	Gly-Glu
	6	196	CGA-TGA	Arg-stop
15	None
16	None
17	None
18	7i	242	CTG-CCG	Leu-Pro
19	None
20	4	111	CTG-CTA	Leu-Leu
	4	131	GGG-GAG	Gly-Glu
	5	152	CCG-CCA	Pro-Pro
	6	208	GAC-AAC	Asp-Asn
	7i	250	CCC-TCC	Pro-Ser
21	6	208	GAC-AAC	Asp-Asn
	7i	248	CGG-TGG	Arg-Trp
22	None
23	None
24	4		6 bp deletion

Table 3 p53 mutation in adjacent non-cancer tissue from esophageal cancer patients.

No.	Exon	Codon	Nucleotide	Amino acid
1	-
2	7i	256	ACA-GCA	Thr-Ala
3	-
4	5	144	CAG-CGG	Gln-Arg
5	None
6	5	145	CTG-CTA	Leu-Leu
7	4	111	CTG-CAG	Leu-Gln
8	4	141	TGC-TGT	Gys-Cys
	7i	256	ACA-GCA	Thr-Ala
9	None
10	None
11	4	107	TAC-TAT	Tyr-Tyr
12	None
13	4	58	TTG-TGG	Leu-Trp
14	None
15	None
16	None
17	5	144	CAG-CGG	Gln-Arg
18	-
19	-
20	None
21	-
22	-
23	7i	267	CCT-TCT	Pro-Ser
24	8	282	CGG-CGC	Arg-Arg