Effect of vector-expressed shRNAs on hTERT expression

Table 1 Sequences of oligonucleotides used in the research.

shRNA	Sequences of oligonucleotides	Target site on hTERT mRNA (GenBank accession no. NM_003219)
1	F: 5’ttcctgcactggctgatgctcgagcatcagccagtgcaggaacttttttg3’	1682-1702nt
	R: 5’aattcaaaaaagttcctgcactggctgatgctcgagcatcagccagtgcaggaa3’
2	F: 5’agtgtctggagcaagttgctcgagcaacttgctccagacactcttttttg3’	1787-1807nt
	R: 5’aattcaaaaaagagtgtctggagcaagttgctcgagcaacttgctccagacact3’
3	F: 5’catggactacgtcgtgggactcgagtcccacgacgtagtccatgcttttttg3’	1958-1978nt
	R: 5’aattcaaaaaagcatggactacgtcgtgggactcgagtcccacgacgtagtccatg3’
4	F: 5’agccacgtctctaccttgctcgagcaaggtagagacgtggctcttttttg3’	2333-2353nt
	R: 5’aattcaaaaaagagccacgtctctaccttgctcgagcaaggtagagacgtggct3’

shRNA encoding cassettes 1-4 were formed by annealing two corresponding oligonucleotides, respectively. F stands for forward and R for reverse. The underlined bases are spacer region and recognition site for XhoI. nt: nucleotide.