Quantitative analysis of tumor mitochondrial RNA using microarray

Table 1 Primer pairs used for PCR-amplified probes of mtRNA.

Functionaldomain	Primer	Sequence	Length (mer)	Tm ( °C)	GC%	Product size (bp)
12SrRNA	F1097	GCCCTAAACCTCAACAGT	18	54.1	50	264
	R1360	CATTTCTTGCCACCTCAT	18	44.5	44.4
16SrRNA	F2618	TAGGGACCTGTATGAATGG	19	48.2	47.4	485s
	R3102	ATAGAAACCGACCTGGAT	18	48	44.4
ND1	F3927	GTCTCAGGCTCAACATC	17	42.1	52.9	273
	R4199	TAGGGTGAGTGGTAGGAA	18	51.1	50
ND2	F5022	CCCACATAGGATGAATAA	18	40.2	38.9	466
	R5487	GCGATGAGGATGGATAGAG	19	46.2	52.6
COI	F6043	TCTAGGTAACGACCACATCTACAAC	25	62.4	44	614
	R6656	CGAAGCCTGGTAGGATAA	18	51.9	50
COII	F7841	TAACAGACGAGGTCAACG	18	50.5	50	351
	R8191	TTGCTCCACAGATTTCAG	18	43.1	44.4
ATPase8	F8366	TGCCCCAACTAAATACTA	18	49.4	38.9	191
	R8556	CAATGAATGAAGCGAACA	18	41.6	38.9
ATPase6	F9000	CGCCTAACCGCTAACATTACTG	22	64.2	50	148
	R9147	AGGCGACAGCGATTTCTA	18	53.8	50
COIII	F9321	CCATAACGCTCCTCATAC	18	47.8	50	203
	R9523	TAGGCTGGAGTGGTAAAA	18	51.8	44.4
ND3	F10200	GCGTCCCTTTCTCCATAA	18	52.4	50	203
	R10402	TTCGGTTCAGTCTAATCCTT	20	51.8	40
ND4	F11581	ATCTGCCTACGACAAACA	18	48.3	44.4	444
	R12024	GTGGTGGGTGAGTGAGCCC	19	61.3	68.4
ND4L	F10573	AATAATACTATCGCTGTTCA	19	45.4	30	189
	R10761	CATTGGAGTAGGTTTAGG	18	46.2	44.4
ND5	F13028	CTGACTCCCCTCAGCCATAGA	21	57.2	57.1	276
	R13303	TGTGGTTGGTTGATGCCG	18	53.3	55.6
ND6	F14322	GTTTACCACAACCACCAC	18	52.1	50	291
	R14612	TCTAAGCCTTCTCCTATTT	19	48.8	36.8
Cyt-b	F15002	GCGCCTCAATATTCTTTATCTGC	23	58.7	43.5	305
	R15306	GAAGGGCAAGATGAAGTGAAA	21	53.4	42.9

ND: NADH dehydrogenase; Cyt-b: Cytochrome b; ATPase: adenosine triphosphatase; CO: cytochrome c oxidase.