Gastric Cancer
Copyright ©2005 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jan 7, 2005; 11(1): 36-40
Published online Jan 7, 2005. doi: 10.3748/wjg.v11.i1.36
Quantitative analysis of tumor mitochondrial RNA using microarray
Cheng-Bo Han, Xiao-Yun Mao, Yan Xin, Shao-Cheng Wang, Jia-Ming Ma, Yu-Jie Zhao
Cheng-Bo Han, Xiao-Yun Mao, Yan Xin, Cancer Institute, First Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning Province, China
Yu-Jie Zhao, Shao-Cheng Wang, Jia-Ming Ma, Biochip Center, China Medical University, Shenyang 110001, Liaoning Province, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No.30371607
Correspondence to: Dr. Cheng-Bo Han, the Fourth Laboratory of Cancer Institute, No.1 Hospital, China Medical University, Shenyang 110001, Liaoning Province, China. hancb@126.com
Telephone: +86-24-23256666-6351
Received: April 10, 2004
Revised: April 15, 2004
Accepted: May 13, 2004
Published online: January 7, 2005
Abstract

AIM: To design a novel method to rapidly detect the quantitative alteration of mtRNA in patients with tumors.

METHODS: Oligo 6.22 and Primer Premier 5.0 bio-soft were used to design 15 pairs of primers of mtRNA cDNA probes in light of the functional and structural property of mtDNA, and then RT-PCR amplification was used to produce 15 probes of mtRNA from one normal gastric mucosal tissue. Total RNA extracted from 9 gastric cancers and corresponding normal gastric mucosal tissues was reverse transcribed into cDNA labeled with fluorescein. The spotted mtDNA microarrays were made and hybridized. Finally, the microarrays were scanned with a GeneTACTM laser scanner to get the hybridized results. Northern blot was used to confirm the microarray results.

RESULTS: The hybridized spots were distinct with clear and consistent backgrounds. After data was standardized according to the housekeeping genes, the results showed that the expression levels of some mitochondrial genes in gastric carcinoma were different from those in the corresponding non-cancerous regions.

CONCLUSION: The mtDNA expression microarray can rapidly, massively and exactly detect the quantity of mtRNA in tissues and cells. In addition, the whole expressive information of mtRNA from a tumor patient on just one slide can be obtained using this method, providing an effective method to investigate the relationship between mtDNA expression and tumorigenesis.

Keywords: Mitochondrial RNA; Gastric cancer; Microarray technique