Protective effect of exogenous adenosine triphosphate on hypothermically preserved rat liver

Table 1 Components of perfusate

Composition	Concentration
Hydroxyethyl starch	50 g/L
Calcium gluconate	80 mmol/L
Raffinose	10 mmol/L
KH2PO4	25 mmol/L
Hydroxyethyl piperazine	10 mmol/L
Dexamethasone	12 mg/L
Penicillin	2×105 units/L
Insulin	100 units/L
1MgCl2	5 mmol/L
1ATP	5 mmol/L

The pH value was modulated to 7.35 with NaOH, and the os-motic pressure was 300-320 mOsm/L;

¹Addition of MgCl₂ and ATP was dependent on the different groups.

Table 2 Energy status in hypothermically preserved rat livers (n = 6, mean ± SD)

	ATP μmol/g wet liver)			EC
	Group A	Group B	Group C	Group A	Group B	Group C
0 h	2.760 ± 0.302	2.760 ± 0.302	2.760 ± 0.302	0.871 ± 0.093	0.871 ± 0.093	0.871 ± 0.093
1 h	2.337 ± 0.202	2.263 ± 0.282	2.514 ± 0.298	0.803 ± 0.090	0.789 ± 0.083	0.791 ± 0.083
2 h	1.914 ± 0.209	1.971 ± 0.205	2.391 ± 0.276a	0.741 ± 0.082	0.736 ± 0.082	0.743 ± 0.079
6 h	1.509 ± 0.211	1.506 ± 0.180	2.523 ± 0.269b	0.653 ± 0.071	0.645 ± 0.068	0.695 ± 0.071a
12 h	1.145 ± 0.177	1.136 ± 0.150	2.715 ± 0.298b	0.543 ± 0.063	0.537 ± 0.060	0.660 ± 0.070b
24 h	0.755 ± 0.082	0.842 ± 0.088	2.547 ± 0.279b	0.380 ± 0.045	0.406 ± 0.045	0.601 ± 0.068b
36 h	0.603 ± 0.065	0.706 ± 0.080	1.782 ± 0.200b	0.316 ± 0.040	0.348 ± 0.042	0.471 ± 0.051b

^aP < 0.05,

^bP < 0.01 vs group A.

Table 3 Activities of AST and LDH in perfusate (IU/L^-.g liver) (n = 6, mean ± SD)

	6 h			12 h			24 h			36 h
	LDH	AST		LDH	AST		LDH	AST		LDH	AST
Group A	80.2 ± 3.8	4.6 ± 0.6	112.7 ± 4.1		8.9 ± 0.9	188.4 ± 5.1		15.6 ± 1.4	452.2 ± 7.4		20.1 ± 1.7
Group B	76.4 ± 4.2	4.8 ± 0.5	123.8 ± 3.6		8.2 ± 0.8	170.1 ± 6.2		14.1 ± 1.7	423.7 ± 6.5		18.7 ± 2.2
Group C	9.3 ± 1.8a	1.2 ± 0.4a		26.7 ± 2.3a	2.4 ± 0.5a		42.6 ± 3.5a	4.4 ± 0.6a		90.1 ± 6.2a	6.3 ± 0.8a

^aP<0.05 vs group A.