Expression of heparanase gene, CD44v6, MMP-7 and nm23 protein and their relationship with the invasion and metastasis of gastric carcinomas

Table 1 Relationship between clinicopathological features and heparanase mRNA expression and CD44v6, nm23 and MMP-7 protein expression in primary gastric carcinomas

	Number of patients
	Heparanase			CD44v6			nm23			MMP-7
	(+)	(-)	P value	(+)	(-)	P value	(+)	(-)	P value	(+)	(-)	P value
Gender			0.307			0.481			0.280			0.415
Female	13	4		12	5		8	9		9	8
Male	16	10		21	5		8	18		17	9
Tumor location			0.216			0.346			0.903			0.804
Upper third	9	1		9	1		4	6		6	4
Middle third	4	3		6	1		3	4		5	2
Lower third	16	10		18	8		9	17		15	11
Gross type			0.242			0.325			0.025			0.121
Borrmann I	1	0		1	0		0	1		1	0
Borrmann II	4	5		5	4		7	2		5	4
Borrmann III	21	9		25	5		9	21		20	10
Borrmann IV	3	0		2	1		0	3		0	3
Histologic type			0.671			0.297			0.444			0.325
Adenocarcinoma	19	11		23	7		13	17		20	10
Mucinous carcinoma	4	1		5	0		1	4		3	2
Signet-ring cell	6	2		5	3		2	6		3	5
carcinoma
Peritoneal metastasis			0.539			1.000			0.045			0.266
Absent	26	14		30	10		13	27		23	17
Present	3	0		3	0		3	0		3	0
Liver metastasis			1.000			1.000			0.133			0.511
Absent	27	14		31	10		14	27		24	17
Present	2	0		2	0		2	0		2	0
Histologic differentiation			1.000			0.704			0.888			0.101
Well/moderate	9	5		10	4		5	9		6	8
Poorly	20	9		23	6		11	18		20	9
Serosal invasion			0.009			0.010			0.101			0.042
Absent	2	6		3	5		5	3		2	6
Present	27	8		30	5		11	24		24	11
Lymph node metastasis			0.018			0.024			0.008			0.084
Absent	7	9		9	7		10	6		7	9
Present	22	5		24	3		6	21		19	8
Distant metastasis			0.039			0.656			0.125			1.000
Absent	21	14		26	9		11	24		21	14
Present	8	0		7	1		5	3		5	3
TNM stage			0.001			0.001			0.228			0.021
I-II	4	10		6	8		7	7		5	9
III-IV	25	4		27	2		9	20		21	8

Table 2 Correlation between the expression of heparanase mRNA and the expression of CD44v6, nm23 and MMP-7 protein

Items	Numberof cases	Heparanase(+)	Correlationcoefficient	P value
CD44v6			-0.440	0.004
(-)	10	3
(+)	33	26
Nm23			-0.021	0.889
(-)	27	18
(+)	16	11
MMP-7			-0.352	0.023
(-)	17	8
(+)	26 21