Anti-liver cancer activity of TNF-related apoptosis-inducing ligand gene and its bystander effects

Table 1 Cell growth-inhibition of SMMC7721 cells

Groups	OD (mean ± SD)	Cell growth-inhibition rate (%)
PBS	1.25 ± 0.20	0
TRAIL	0.11 ± 0.02ac	91.2
Bax	0.65 ± 0.13a	48.0
LacZ	0.89 ± 0.04	28.8

^aP < 0.05 vs PBS,

^cP < 0.05 vs Bax.

Table 2 Cell growth-inhibition of NHFB cells

Groups	OD (mean ± SD)	Cell growth-inhibition rate (%)
PBS	0.66 ± 0.02	0
TRAIL	0.62 ± 0.02c	6.1
Bax	0.36 ± 0.02a	45.5
LacZ	0.61 ± 0.03	7.6

^aP < 0.05 vs PBS,

^cP < 0.05 vs Bax.

Table 3 Percentage of apoptotic SMMC7721 cells

Groups	Percentage of apoptotic cells (%, mean ± SD)
TRAIL	29.07 ± 4.96ace
Bax	12.53 ± 1.23ae
LacZ	6.58 ±0.49ac
PBS	2.94 ± 0.63ce

^aP<0.05 vs PBS,

^cP<0.05 vs Bax,

^eP<0.05 vs LacZ.

Table 4 Bystander effects of TRAIL on SMMC7721 cells

Percentage ofSMMC7721/TRAIL (%)	OD (mean ± SD)	Cell growth-inhibitionrate (%)
0	0.693 ± 0.028	0
5	0.583 ± 0.036	15.9
25	0.228 ± 0.014	67.0
50	0.137 ± 0.019	80.2
75	0.094 ± 0.009	86.4
100	0.084 ± 0.010	87.7

Table 5 Percentage of apoptotic SMMC7721 cells

Groups	OD (mean ± SD)	Cell growth-inhibition rate (%)
PBS	0.846 ± 0.016	0
Cultured medium	0.794 ± 0.027	4.0
TRAIL	0.108 ± 0.010a	87.5

^aP<0.05 vs PBS.