Differentially expressed genes between solitary large hepatocellular carcinoma and nodular hepatocellular carcinoma

Table 1 Primer sequences and annealing temperature for PCR

Gene		Primer sequences	Product size	Annealing temperature
RhoC	Up	5’-TCCTCATCGTCTTCAGCAAG-3’	183 bp	56 °C
	Down	5’-CTGCAATCCGAAAGAAGCTG-3’
protocadherin	Up	5’-GAAGCTTCAAGCTATGAAAA-3	256 bp	52 °C
LKC	Down	5’-CCTTGATTTCCTGACTGTTC-3’
β2-MG	Up	5’-ACCCCCACTGAAAAAGATGA-3’	120 bp	56 °C
	Down	5’-ATCTTCAAACCTCCATGATG-3’

Table 2 Numbers of variably expressed genes in SLHCC and NHCC

Class	No. of up-regulated genes	No. of down-regulated genes
Oncogenes and antioncogenes	6	5
Genes encoding proteins related to ionic channel and protein transportation	7	7
Genes encoding cellular cycle modulator	3	9
Genes associated with cellular stress	1	1
Genes associated with cellular skeleton and movement	5	7
Genes related to cellular apoptosis	2	2
Gene encoding proteins regulating synthesis, repair, and rearrangement of DNA	0	4
Genes encoding transcription factors and proteins binding with DNA	17	6
Cytokine receptor gene	4	3
Immunological protein gene	18	17
Genes associated with cellular metabolism	32	32
Genes encoding signal transductional factor	39	31
Genes encoding factors that regulated protein translation	11	37
Genes encoding proteins that were consistent with body growth and development	7	8
Other	203	144