Brief Reports
Copyright ©The Author(s) 2004.
World J Gastroenterol. Dec 1, 2004; 10(23): 3514-3517
Published online Dec 1, 2004. doi: 10.3748/wjg.v10.i23.3514
Table 1 Sequences of primers and probes used in this study
Gene and oligonucleotideSequencePCR product size (bp)
GAPDH
Upper primer5’-GAAGGTGAAGGTCGGAGTC-3’
Lower primer5’-GAAGATGGTGATGGGATTTC-3’226
Probe5’-(FAM) CAAGCTTCCCGTTCTCAGCC (TAMRA)-3’
hTERT
Upper primer5’-TGACACCTCACCTCACCCAC-3’
Lower primer5’-CACTGTCTTCCGCAAGTTCAC-3’95
Probe5’-(FAM) ACCCTGGTCCGAGGTGTGTCCCTGA (TAMRA)-3’
Table 2 Relationship between hTERT mRNA expression level and clinicopathological parameters in patients with gastric cancer
ParameternNhTERT
P
Low (n = 17)High (n = 18)
Age (yr)
< 501376
≥ 502210120.73
Gender
Male251312
Female10460.71
Size of diameter (cm)
< 519118
≥ 5166100.31
Location
Cardia1486
Body and antrum Degree of differentiation219120.50
Well and moderately differentiated16124
Poorly differentiated and undifferentiated195140.007
Lymph node metastasis
N0844
N11385
N214590.40
Depth of invasion
T1 and T2241311
T3 and T411470.43
Metastasis
M020128
M1155100.17