Epstein-Barr virus in hepatocellular carcinogenesis

Table 1 Primer sequences and amplification programs of PCR and RT-PCR detection

Transcript	Product size	Genomic coordinates in GenBank	Primer sequence (5‘-3‘)	Amplification program
EBV	337 bp	B95-8	GAGTGTGTGCCAGTTAAGGT (168 056-16 8075 20 bp)	94 °C 3 min (94 °C 30 s 55 °C
LMP1[1]		V01555	CTAGCGACTCTGCTGGAAAT (168 373-16 8392 20 bp)	40 s 72 °C 1 min) × 40 72°C 8 min
EBV	130 bp	B95-8	CATCACCGTCGCTGACT (14 525-14 541 17 bp)	95 °C 3 min (94 °C 30 s 52 °C
BamHI W		V01555	GTTGGGCTTAGCAGAAA(14 638-14 654 17 bp)	40 s 72 °C 45 s) × 40, 72°C 7 min
HBV	198 bp	HBV	AGGTATGTTGCCCGTTTGT (457-475 19 bp)	95 °C 5 min (94 °C 30 s 52 °C
S gene		AB073856	GAGGCCCACTCCCATAGG (637-654 18 bp)	40 s 72 °C 50 s) × 35, 72°C 7 min
HBV	232 bp	HBV	TGTGCTGCCAACTGGATCCT (1 387-1 406 20 bp)	95 °C 3 min (94 °C 30 s 55 °C
X gene		AB073856	GTGGTCTCCATGCGACGTG (1600-1618 19 bp)	40 s 72 °C 45 s) × 37 72°C 7 min
HCV	165 bp	HCV	GCGGAACCGGTGAGTACAC (138-156 19 bp)	95 °C 5 min (94 °C 30 s 55 °C
cDNA		NC_001433	GCACTCGCAAGCACCCTATC (283-302 20 bp)	40 s 72 °C 50 s) × 40 72°C 7 min
Outer
HCV	142 bp	HCV	GGAATTGCCAGGACGACC (158-175 18 bp)	95 °C 5 min (94 °C 30 s 55 °C
cDNA		NC_001433	CTCGCAAGCACCCTATCAG (281-299 19 bp)	40 s 72 °C 50 s) × 40 72°C 7 min
Inner
HDV	216 bp	HDV	GCGGGTCCGTCGTTCCAT (653-670 18 bp)	95 °C 5 min (94 °C 30 s 55 °C
cDNA		AB037947	GTCCGACCTGGGCATCCGT (850-868 19 bp)	40 s 72 °C 50 s) × 35 72°C 7 min
Outer
HDV	114 bp	HDV	TCGAGAATCGGCAAATG (752-768 17 bp)	95 °C 5 min (94 °C 30 s 52 °C
cDNA		AB037947	GACCTGGGCATCCGTAAG (847-864 18 bp)	40 s 72 °C 50 s) × 35 72°C 7 min
Inner
β -globulin	210 bp	Beta-globin	ACACAACTGTGTTCACTAGC (120-139 20 bp)	94 °C 5 min (94 °C 30 s 55 °C
(PCO3-PCO5)[2]		human	GAAACCCAAGAGTCTTCTCT (310-329,20bp)	40 s 72 °C 1 min) × 35 72°C 8 min
		DNA AY128650