Honokiol induces apoptosis through p53-independent pathway in human colorectal cell line RKO

Table 1 Expected size of PCR amplification product with each apoptosis modulator primer pair

Apoptosis modulator	Upstream primer (5’-3’)	Downstream primer (5’-3’)	Size (bp)
Bax	ACCAAGAAGCTGAGCGAGTGT	ACAAACATGGTCACGGTCTGC	332
Bcl-xl	GGAGCTGGTGGTTGACTTTCT	CCGGAAGAGTTCATTCACTAC	379
Bid	GAC CCG GTG CCT CAG GA	ATG GTC ACG GTC TGC CA	586
Bcl-2	TTGTGGCCTTCTTTGAGTTCG	TACTGCTTTAGTGAACCTTTT	332
β -actin	AGGCCAACCGCGAGAAGATGACC	GAAGTCCAGGGCGACGTAGCAC	350

Table 2 Effect of honokiol on cell cycle distribution and apoptosis in RKO cells

Treatment(μg/mL)	Cell cycle distribution (%)			Apoptosis (%)
	G0/G1	S	G2/M
Control	44.76	45.09	10.14	0.21
5	48.03	42.37	9.60	4.51
10	54.04	40.30	5.66	14.10b
15	58.94	39.36	1.70	20.31d

Cell cycle distribution was determined after 48 h of treatment in each group. The tabulated percentages were an average calculated on the results of three separate experiments.

^bP < 0.01,

^dP < 0.01 vs corresponding control group.