Expansion and activation of natural killer cells from PBMC for immunotherapy of hepatocellular carcinoma

Table 1 Proportion of CD16+CD56+ cells compared to other cell groups after NK cell expansion cultures

Irradiated target cells used for expansion culture	Cell proportion in lymphocyte populations (%)
	CD3+CD56-	CD3-CD56+	CD3+CD56+	CD16+CD56+
HFWT	15.2	72.0	12.3	72.7
HCC-2	49.7	18.5	31.1	22.8

Table 2 Proportion of CD16+CD56+ after co-culture of PBMC with irradiated HFWT cells with and without separation by a membrane filter

PBMCs and HFWT co-culture conditions	Cell proportion in final lymphocyte populations (%)
	CD3+CD56-	CD3-CD56+	CD16+CD56+
Without separation	13.4	78.2	71.7
Separated by a membrane filter	83.4	5.3	3.9