Copyright
©The Author(s) 2004.
World J Gastroenterol. May 15, 2004; 10(10): 1508-1512
Published online May 15, 2004. doi: 10.3748/wjg.v10.i10.1508
Published online May 15, 2004. doi: 10.3748/wjg.v10.i10.1508
Table 1 PCR condition and restriction enzymes
Genes | Primers | Annealing temp | RE |
CYP7A[16,17] | 5’TGGTAGGTAAATTATTAATAGATGT3’ | ||
5’AAATTAAATGGATGAATCAAAGAGC3’ | 61°C | Bsa I | |
apo B[10] | 5’GGAGACTATTCAGAAGCTAA3’ | ||
5’GAAGAGCCTGAAGACTGACT3’ | 60°C | Xba I | |
apo E[24] | 5’ACAGAATTCGCCCCGGCCTGGTACAC3’ | ||
5’TAAGCTTGGCACGGCTGTCCAAGGA3’ | 60°C | Hha I | |
LDL receptor[25] | 5’CACTTTGTATATTGGTTGAAACTGT3’ | ||
5’CACTGAACAAATACAGCAACCAGGG3’ | 62°C |
Table 2 Demographic characteristics and biochemistry for patients and controls
Patients | Controls | P value | P value1 | |
No. (male/female) | 105 (78/27) | 274 (184/90) | ||
Age (yr) | 47.53 ± 10.98 | 47.94 ± 12.21 | ||
BMI (kg/m2) | 24.47 ± 3.09 | 23.50 ± 2.16 | < 0.01 | |
Triglycerine (mmol/L) | 1.32 ± 1.15 | 1.19 ± 0.82 | ||
Cholesterol (mmol/L) | 4.66 ± 0.92 | 4.91 ± 0.96 | < 0.05 | < 0.01 |
HDL (mmol/L) | 1.33 ± 0.36 | 1.40 ± 0.34 | ||
LDL (mmol/L) | 2.56 ± 0.67 | 2.69 ± 0.73 | < 0.05 | |
apo A I (g/L) | 1.34 ± 0.21 | 1.39 ± 0.18 | < 0.05 | |
apo B (g/L) | 1.00 ± 0.22 | 1.03 ± 0.24 | < 0.05 |
Table 3 Distribution of genotypes for patients and controls
Genes | Genotypes | Patients (%) | Controls (%) |
CYP7A | AA | 44 (41.90) | 79 (28.83) |
AC | 44 (41.90) | 140 (51.10) | |
CC | 17 (16.20) | 55 (20.07) | |
Apo B | X+/+ | 1 (0.95) | 0 (0) |
X+/- | 16 (15.24) | 22 (8.03) | |
X-/- | 88 (83.81) | 252 (91.97) | |
Apo E | ε2/2 | 0 (0) | 1 (0.36) |
ε2/3 | 15 (14.29) | 45 (16.42) | |
ε2/4 | 5 (4.76) | 6 (2.19) | |
ε3/3 | 73 (69.52) | 183 (66.80) | |
ε3/4 | 11 (10.48) | 37 (13.50) | |
ε4/4 | 1 (0.95) | 2 (0.73) | |
LDL receptor | AA | 41 (39.05) | 90 (32.85) |
AB | 2 (1.90) | 13 (4.74) | |
AC | 32 (30.48) | 104 (37.96) | |
BB | 3 (2.85) | 4 (1.46) | |
BC | 8 (7.62) | 22 (8.03) | |
BC | 19 (18.60) | 41 (14.96) |
Table 4 Frequency of alleles for patients and controls (%)
Table 5 Association of CYP7A gene polymorphism with plasma lipids
Patients | Controls | All | ||||
AA/AC | CC | AA/AC | CC | AA/AC | CC | |
No. | 88 | 17 | 219 | 55 | 307 | 72 |
Triglyceride (mmol/L) | 1.25 ± 0.99 | 1.70 ± 7.77 | 1.15 ± 0.82 | 1.36 ± 0.81 | 1.18 ± 0.87 | 1.44 ± 1.11 |
Cholesterol (mmol/L) | 4.64 ± 0.95 | 4.78 ± 0.79 | 4.84 ± 0.94c | 5.16 ± 0.99c | 4.78 ± 0.95g | 5.07 ± 0.95g |
HDL (mmol/L) | 1.32 ± 0.37a | 1.35 ± 0.32 | 1.42 ± 0.34a | 1.33 ± 0.72 | 1.39 ± 0.35 | 1.33 ± 0.32 |
LDL (mmol/L) | 2.56 ± 0.70 | 2.59 ± 0.45e | 2.63 ± 0.72c | 2.91 ± 0.72e,c | 2.61 ± 0.71g | 2.83 ± 0.67g |
Apo AI (g/L) | 1.34 ± 0.22a | 1.36 ± 0.15 | 1.39 ± 0.18a | 1.36 ± 0.20 | 1.38 ± 0.19 | 1.36 ± 0.18 |
Apo B (g/L) | 1.00 ± 0.23 | 1.02 ± 0.15 | 1.01 ± 0.24c | 1.10 ± 0.23c | 1.01 ± 0.23g | 1.08 ± 0.22g |
Table 6 Stepwise logistic regression of multiple variables
- Citation: Jiang ZY, Han TQ, Suo GJ, Feng DX, Chen S, Cai XX, Jiang ZH, Shang J, Zhang Y, Jiang Y, Zhang SD. Polymorphisms at cholesterol 7α-hydroxylase, apolipoproteins B and E and low density lipoprotein receptor genes in patients with gallbladder stone disease. World J Gastroenterol 2004; 10(10): 1508-1512
- URL: https://www.wjgnet.com/1007-9327/full/v10/i10/1508.htm
- DOI: https://dx.doi.org/10.3748/wjg.v10.i10.1508