Expression and altered subcellular localization of the cyclin-dependent kinase inhibitor p27Kip1 in hepatocellular carcinoma

Table 1 Clinicopathologic characteristics of 32 Patients

Clinicopathologic parameters	Number	%
Age
≤ 45 years	14	43.8
> 45 years	18	56.2
Gender
Male	26	81.2
Female	6	18.8
Gross type
Massive	15	46.9
Nodular	17	53.1
Tumor size
≤ 5 cm	5	15.6
> 5 cm	27	84.4
Cellular differentiation
I	4	12.5
II	17	53.1
III	5	15.6
IV	6	18.8
Degree of invasion
T1 + T2	12	37.5
T3 + T4	20	62.5
Stage
I + II	10	31.2
III + IV	22	68.8
Lymph node metastasis
Present	4	87.5
Absent	28	12.5
Portal invasion
With	3	9.4
Without	29	90.6
Infiltration
Present	12	37.5
Absent	20	62.5

Table 2 Relationship among p27, PCNA, and cytoplasmic p27staining with clinicopathologic features

Parameters	p27 LI (%)	P	PCNA LI (%)	P	Cytoplasmic p27 LI (%)	P
Gross type
Massive	20.5 ± 13.1	0.037	46.4 ± 13.4	0.249	25.9 ± 18.9	0.221
Nodular	35.7 ± 23.4		52.3 ± 14.7		17.9 ± 17.3
Tumor size
≤ 5 cm	23.8 ± 14.4	0.645	38.1 ± 10.7	0.047	27.0 ± 24.1	0.481
> 5 cm	28.4 ± 20.9		51.7 ± 13.9		20.6 ± 17.4
Cellular differentiation
Well or moderate	29.7 ± 21.4	0.438	45.7 ± 13.8	0.031	23.2 ± 18.0	0.498
Poor	23.8 ± 17.1		56.9 ± 12.6		18.6 ± 19.1
Degree of invasion
T1 + T2	32.6 ± 23.3	0.285	51.1 ± 14.7	0.64	33.0 ± 22.3	0.019
T3 + T4	24.7 ± 17.5		48.6 ± 14.4		14.8 ± 11.1
Stage
I + II	33.6 ± 25.6	0.261	48.0 ± 12.9	0.688	35.3 ± 23.9	0.029
III + IV	24.9 ± 16.7		50.2 ± 15.0		15.4 ± 10.7
Infiltration
Present	18.3 ± 18.8	0.036	44.4 ± 12.3	0.117	19.0 ± 18.3	0.536
Absent	33.3 ± 18.7		52.6 ± 14.7		23.2 ± 18.5
Lymphocyte metastasis
With	25.5 ± 2.6	0.565	56.0 ± 14.9	0.339	16.0 ± 10.6	0.518
Without	27.9 ± 21.3		48.6 ± 14.2		22.4 ± 19.1
Portal invasion
Present	14.3 ± 12.7	0.229	58.3 ± 12.0	0.267	13.0 ± 11.8	0.398
Absent	29.1 ± 20.1		48.6 ± 14.3		22.5 ± 18.7

Table 3 Univariate and multivariate analyses of individual parameters

Variables	P values
	Univariate	Multivariate
Age	0.150	0.016
Tumor size	0.513	0.678
Carcinoma differentiation	0.415	0.628
Stage	0.200	0.080
Lymphocyte metastasis	0.821	0.050
Infiltration	0.317	0.849
Portal invasion	0.021	0.619
p27	0.033	0.009
Cytoplasmic p27	0.114	0.624
PCNA	0.915	0.282