Abstracts Open Access
Copyright ©The Author(s) 2000. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 15, 2000; 6(Suppl3): 42-42
Published online Sep 15, 2000. doi: 10.3748/wjg.v6.iSuppl3.42
The function of apoptosis and protein expression of Bcl-2, p53 and C-myc in the development of gastric cancer
An-Gao Xu, Shao-Guang Li, Ji-Hong Liu, Ai-Hua Gan, Research Laboratory of Digestive Diseases, Huizhou Central People’s Hospital, Huizhou 516001, Guangdong Province, China
Author contributions: All authors contributed equally to the work.
Supported by the Medical Research Foundation of Guangdong Province, No. 1997423
Correspondence to: Dr. An-Gao Xu, Research Laboratory of Digestive Diseases, Huizhou Central People’s Hospital, No. 41 Elingbeilu Road, Huizhou 516001, Guangdong Province, China
Telephone: 752-2288160 Fax: 752-2122977
Received: May 25, 2000
Revised: June 29, 2000
Accepted: July 10, 2000
Published online: September 15, 2000

Abstract

AIM: To understand the rule and possible function of apoptosis and protein expression of Bcl-2, p53 and C-myc in chronic gastritis, gastric ulcer, non-class nonproliferation of gastric mucosa and gastric cancer.

METHODS: Apoptosis was detected by using in situ terminal labelling (TUNEL). The protein expression of Bcl-2, p53 and C-myc was detected by immunohistochemical method.

RESULTS: The indexes of apoptosis in chronic active gastritis, gastric ulcer, mild and severe non-classic proliferation of gastric mucosa, early and progressive gastric cancer were 16.8% ± 12.3%, 24.1% ± 20.0%, 19.3% ± 16.4%, 15.7% ± 15.2%, 10.1% ± 9.1% and 6.3% ± 6.0%, respectively. The index of progressive gastric cancer was lower than that of early gastric cancer and non-classic proliferation of gastric mucosa (P < 0.05). The positive rate of Bcl-2 protein was 9.4%, 27.6%, 52.9%, 75.0%, 83.3% and 46.7%, respectively. The positive rate of Bcl-2 of early gastric cancer was higher than that of progressive gastric cancer. The positive rates of p53 protein of severe non-classic proliferation, early and progressive gastric cancer were 25.0%, 33.3% and 63.3%, respectively. The positive rate of p53 of progressive gastric cancer was higher than that of early gastric cancer and non-classic proliferation (P < 0.05). In Lauren types, the index of apoptosis, protein expression rates of Bcl-2, p53 and C-myc of intestinal type were 8.3% ± 7.2%, 38.9%, 77.7% and 56.6%, while that of diffuse type were 5.1% ± 4.9%, 58.3%, 50.0% and 8.3%, respectively. All markers had statistical difference between two types (P < 0.05).

CONCLUSION: Apoptosis was inhibited stepwise in the development of non-classic proliferation of gastric mucosa to early gastric cancer and then to progressive gastric cancer. The high expression of Bcl-2, p53 and C-my c was related to the development of gastric cancer. Bcl-2 might play an important role in early gastric cancer while p53 and C-myc act mostly in middle and late stage gastric cancer. The Lauren typing of gastric cancer is closely related to the index of apoptosis and expression of Bcl-2, p53 and C-myc. the Medical Research Foundation of Guangdong Province, No. 1997423

Key Words: Stomach neoplasms; Genes, p53; Apoptosis; Gastric mucosa; Immunohistochemistry; Genes, myc

Footnotes

E- Editor: Hu S

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