Abstracts Open Access
Copyright ©The Author(s) 2000. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 15, 2000; 6(Suppl3): 24-24
Published online Sep 15, 2000. doi: 10.3748/wjg.v6.iSuppl3.24
Effect of pentagastrin on IL-1β induced inhibition of insulin secretion in neonatal rat islets of Langerhans
Ji-Tian Xu, Ming Yan, Yun-Wei Yao, Ren Wu, Department of Physiology, Henan Medical University, Zhengzhou 450052, Henan Province, China
Author contributions: All authors contributed equally to the work.
Supported by the Science Fund of Henan Province, No. 981170811
Correspondence to: Dr. Ji-Tian Xu, Department of Physiology, Henan Medical University, Zhengzhou 450052, Henan Province, China
Telephone: 371-6973648
Received: November 16, 1999
Revised: April 5, 2000
Accepted: May 10, 2000
Published online: September 15, 2000

Abstract

AIM: To observe the effect of pentagastrin (G-5) on IL-1β induced inhibition of insulin secretion in newborn rat islet of Langerhans.

METHODS: Islets of Langerhans of 3 to 5 day old rats were isolated by collagenase digestion. The islets were maintained free floating in culture medium RPMI-1640, containing 10% (V/V) calf serum, and distributed randomly in 96-well plastic plates (6 wells in each group). There are 15 is lets per well in 0.2 mL culture medium. The islets were kept at 37 °C in mixed gases of 5% CO2 and 95% humidified air for the time required by the experimental design. Three experiments were performed in this study. (1) IL-1β induced inhibition of insulin secretion in isolated islets of Langerhans. (2) Effect of G-5 on IL-1β induced inhibition of insulin secretion. And (3) Effect of G-5 on the functional repair of islet B-cells inhibited by IL-1β. Accumulated and glucose stimulated insulin secretion was measured by radioimmunoassay in all studies. Data are presented as -x±s. Differences between groups were analyzed using the Student’s t test. P < 0.05 was considered significant.

RESULTS: The function of islet B cells, which has been received IL-1β treated for 24 h, was dose-dependently inhibited. The accumulated and glucose stimulated insulin secretion was significantly lower than that of the control group (P < 0.05). The inhibitory effect of IL-1β on islet B cells can be partially reversed by G 5. Accumulated and stimulated insulin secretion of G-5 0.6 ng/mL and 0.8 ng/mL groups was significantly higher than that of IL-1β treated alone group (P < 0.05). The function of islet B-cells, which received IL-1β treatment for 24 h, could partially recover after G-5 treatment for another 24 h. But accumulated and glucose stimulated insulin secretion in groups with G-5 treatment for 10 h groups had no significant difference as compared with IL-1β treated alone group (P > 0.05).

CONCLUSION: The present results indicate that G-5 may have a protective effect against the toxicity of IL-1β on islet B-cells.

Key Words: Pentagastrin; Interleukin-1; Islet, Langerhans; Insulin; Diabetes mellitus, insulin-dependent



Footnotes

E- Editor: Hu S

$[References]