Abstracts Open Access
Copyright ©The Author(s) 2000. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 15, 2000; 6(Suppl3): 136-136
Published online Sep 15, 2000. doi: 10.3748/wjg.v6.iSuppl3.136
Development of somatostatin and bombesin-secreting cells of proventriculus from Shao ducks
Yuan-Xin Shen, Jiu-Sheng Wu, College of Animal Science, Zhejiang University, Hangzhou 310029, Zhejiang Province, China
Ying Gao, Hangzhou 2nd Hospital, Hangzhou 310015, Zhejiang Province, China
Author contributions: All authors contributed equally to the work.
Supported by The Natural Science Foundation of China, No. 39070634, 39670546
Correspondence to: Dr. Yuan-Xin Shen, College of Animal Science, Zhejiang University, 268 Kaixuan Road, Hangzhou 310029, Zhejiang Province, China
Received: February 22, 2000
Revised: March 20, 2000
Accepted: May 10, 2000
Published online: September 15, 2000

Abstract

AIM: To study the development of D (somatostatin secreting) and P (bombesin secreting) cells of proventriculus from Shao ducks at different ages.

METHODS: Ninety Shao ducks were divided into nine groups, 10 ducks per group. The ducks were slayed group by group at the nine time points of week 0 (after hatching), 1, 2, 4, 6, 10, 14, 18 and 22. Proventriculus samples from each duck were collected, fixed by Bouin solution and embedded with paraffin. Sections were made and stained with an avidin-biotin peroxidase complex kit (Dako Co. Ltd.) to visualize D and P cells of each proventriculus. Thirty glandular lobes per duck were observed for enumeration of D and P cells.

RESULTS: (1) Both D and P cells were mostly oval or polygonal shape with dumpy cytoplasmic processes and located in the inner and central area of the glandular lobe. (2) The D and P cells peaked at week 18 and 6 respectively. And (3) there was no apparent correlation between D cells and the body weight until week 4. Negative correlation was observed from week 6 and reached a marked level at 18 wk (r = -0.829, P < 0.05). Individuals with max imum body weight had less D cells than those with minimum body weight from 10-18 wk.

CONCLUSION: The morphology and distribution of D and P cells in the roventriculus of Shao ducks were similar to Peking ducks, gooses and chickens. Both D and P cells had continuing development during the postnatal period, though the rates of their development were different. If appeared that beyond a definite number of D cells, they were negatively correlated with the body weight.

Key Words: Somatostatin; Caerulein; Immunohistochemistry; D cells; P cells; Ducks



Footnotes

E- Editor: Zhang FF

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