Relation between N-Nitrosos compound and gastric cancer - establishment of a model system and research for human gastric cancer

doi:10.3748/wjg.v6.iSuppl3.116

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Sep 15, 2000; 6(Suppl3): 116-116
Published online Sep 15, 2000. doi: 10.3748/wjg.v6.iSuppl3.116

Relation between N-Nitrosos compound and gastric cancer - establishment of a model system and research for human gastric cancer

Xiu-Lan Su, Yang Ke

Xiu-Lan Su, Department of cytochemistry, Inner Mongolia Medical College, Huhot 010059, Inner Mongolia, China

Yang Ke, Beijing Institute for Cancer Research, Beijing 100034, China

Author contributions: All authors contributed equally to the work.

Correspondence to: Xiu-Lan Su, Department of cytochemistry, Inner Mongolia Medical College, No. 5 Xin Hua Street, Huhot 010059, Inner Mongolia, China

Received: May 12, 2000
Revised: June 27, 2000
Accepted: July 10, 2000
Published online: September 15, 2000

Abstract

AIM: To establish a model system for studying gastric carcinogenesis of MNNG, a gastric cancer related carcinogen.

METHODS: Cell culture transformation, PCR restriction fragment length polymorphism (PCR-RFLP), DNA blotting and immunochemical techniques and analysis of LDH isozyme and chromosome were performed.

RESULTS: GES-1 cells surviving by MNNG treatment were named MC (2 × 10⁵ M for 24 h) and MC B (2 × 10⁷ M for 7 d). The two cell lines treated by MNNG showed more malignant than maternal cell GES-1 with the evidences of more chromosome aberrations, abnormal morphology and cytoskeleton and also gained the ability of colony formation on soft agar. C-Ha-ras gene point mutation in the 12^th codon and LDH isoenzyme abnormal express were found in MC-B cells. In addition, C-met gene rearrangement was revealed by Southern blot analysis in MC-B and MC.

CONCLUSION: This gastric epithelial cell system is an important model system for further study of stomach cancer, MNNG had a selective effect on the cytoskeleton microfilament in human gastric epithelial cells and intimately associated with the activation of certain oncogenes and some protein.

Key Words: Stomach neoplasms; Nitroso compounds; Carcinogens; Polymorphism; Polymerase chain reaction; Immunohistochemistry; Incidence

Citation: Su XL, Ke Y. Relation between N-Nitrosos compound and gastric cancer - establishment of a model system and research for human gastric cancer. World J Gastroenterol 2000; 6(Suppl3): 116-116
URL: https://www.wjgnet.com/1007-9327/full/v6/iSuppl3/116.htm
DOI: https://dx.doi.org/10.3748/wjg.v6.iSuppl3.116

Footnotes

E- Editor: Hu S