Overproduction of nitric oxide inhibits vascular reactivity in portal hypertensive rats

Abstract

AIM: To evaluate the relationship between nitric oxide (NO) and hyperdynamic circulatory status in portal hypertension.

METHODS: Twenty male Sprague Dawley rats (weighing 200 ± 20 g) were randomized into two groups: portal hypertension group (n = 12) and sham-operated control group (n = 8). The portal hypertensive model was established by means of graded constriction of the portal vein. The concentrations of nitrite (NO₂^-) in the portal vein and peripheral blood were measured by fluorometric assay to reflect NO levels. The reactivity of isolated abdominal aortic rings from rats with partial portal vein constriction and controls was determined by assessing response to administration of potassium chloride (KCl) (10-80 mmol/L) and phenylephrine (10^-9-10^-4 mol/L) with or without preincubation with NO synthase inhibitor Nω-nitro-L-arginine (L-NNA).

RESULTS: Serum concentrations of NO₂^- in the portal vein blood (0.766 ± 0.097 μmol/L) and peripheral blood (0.687 ± 0.092 μmol/L) were elevated in portal hypertensive rats, as compared with the concentrations in controls (0.613 ± 0.084 μmol/L and 0.591 ± 0.045 μmol/L respectively, both P < 0.01). In addition, the rates of NO₂^- in portal vein blood were markedly higher than those in peripheral blood (P < 0.05) in the portal hypertensive rats. Abdominal aortic rings from rats with portal vein constriction exhibited significantly impaired contractility to phenylephrine and KCl, as compared with the control rats. The EC₅₀ values of KCl were markedly higher in the portal hypertensive rings (26.5 ± 0.9 mmol/L) than in the control rings (22.3 ± 1.7 mmol/L, P < 0.01), as were the EC₅₀ values of phenylephrine (37.2 ± 0.4 nmol/L vs control rings: 28.1 ± 0.2 nmol/L, P < 0.01). After preincubation of rings with L-NNA, the difference in EC₅₀ values between portal hypertensive and control rings was no longer statistically significant for either KCl (20.18 ± 0.8 mmol/L vs 19.4 ± 1.2 mmol/L, P > 0.05) or phenylephrine (22.4 ± 1.8 nmol/L vs 21.8 ± 1.4 nmol/L, P > 0.05). However, the maximal concentrations of KCl and phenylephrine for inducing contractions were still significantly lower in the portal hypertensive rings (1.08 ± 0.1 g and 1.43 ± 0.14 g) than in the control rings (1.21 ± 0.11 g and 1.72 ± 0.11 g respectively, both P < 0.05). Thus, addition of the NO synthase inhibitor L-NNA could partially restore contractile responses to KCl and phenylephrine in portal hypertensive rings.

CONCLUSION: NO overproduction inhibits the vascular reactivity to vasoconstrictors, and it might be one of the main causes of vasodilatation and hyperdynamic circulatory status in portal hypertension.

Key Words: Portal hypertension; Nitric oxide; Vascular reactivity; Hyperdynamic circulatory status; Vasodilatation

INTRODUCTION

Mechanisms of portal hypertension are complicated. In addition, circulatory abnormalities may affect the portal venous systemic circulation regardless of the presence or absence of defective liver structure and function. Hyperdynamic circulation can perpetuate and aggravate portal hypertension, possibly resulting in a series of grave cardiovascular and non-cardiovascular complications[1]. In recent years, nitric oxide (NO), a newly identified neurotransmitter, cell-killing factor and cellular messenger molecule, has attracted the attention of biologists and medical workers. Evidence has shown that NO plays an important role in cardiovascular function and is involved in the physiology of vascular tone, blood flow velocity and vascular resistance. Recent studies have also indicated that NO is involved in the pathophysiology aof portal hypertension[2].

In order to evaluate the relationship between NO and hyperdynamic circulation in portal hypertension, we used rats with partial portal vein constriction and rats that were sham-operated (controls) to investigate the reactivity of isolated vascular rings to potassium chloride (KCl) and to phenylephrine with or without pre-exposure to the NO synthetic inhibitor Nω-nitro-L-arginine (L-NNA). We determined that the serum concentrations of NO₂^- detected by fluorometric analysis reflect NO levels. The results of this study provide new insights that may help towards developing new treatments of portal hypertension.

MATERIALS AND METHODS

Animal model

Twenty male Sprague-Dawley rats (weighing 200 ± 20 g) were randomized into two groups: the portal hypertension model group (n = 12) and the sham-operated control group (n = 8). The portal hypertensive model was established by means of graded constriction of the portal vein. Specifically, after the rats were anesthetized with ketamine hydrochloride (0.15 mg/100 g, intra-abdominal), the portal vein was isolated and a stenosis was created by means of a single ligature (3-0 silk) placed around both the portal vein trunk and a needle (blunt, external diameter 0.8 mm). Removal of the needle facilitated production of a consistent extrahepatic portal hypertension. The sham operation consisted of dissection and visual inspection of the portal vein.

Sample preparation

Two weeks after the surgeries, the animals were re-anesthetized and portal venous pressure was measured. Blood samples from the portal vein and abdominal vein (2.0-3.0 mL) were obtained by direct puncture and centrifuged at 3000 rpm for 10 min to separate the serum. The serum samples were then stored at -40 °C for subsequent detection of NO₂^-. Whole blood samples (1.5 mL) were also obtained for other assays to detect serum electrolytes and markers of liver function. The abdominal aorta was dissected, placed in oxygenated Krebs solution, stripped of all fatty tissues and adventitia, and then cut into 3 mm rings. the length and external diameter of which were measured.

Observation of vascular reactivity

The prepared rings from both groups of animals were mounted horizontally between two stainless-steel stirrups in individual organ bath chambers filled with 10 mL of modified Krebs solution (mmol/L concentrations: NaCl 118.3, KCl 14.7, NaHCO₃ 25, MgSO₄ 1.2, KH₂PO₄ 1.2, CaCl₂ 2.5, glucose 11.1, and EDTA 0.026, pH 7.4) and constantly bubbled with a mixture of 95% O₂ and 5% CO₂ and maintained at 37 °C. After 60 min of equilibration, the rings were allowed to adjust gradually with 30 mmol/L KCl until the optimal resting tension was obtained. All the experiments were the performed on the rings in the optimal resting tension, which was approximately 1.5 g. The contraction responsiveness to cumulative additions of KCl (10, 20, 30, 40, 50, 60, 70 and 80 mmol/L) and phenylephrine (10^-9, 10^-8, 10^-7, 10^-6, 10^-5 and 10^-4 mol/L) were measured with force displacement transducers (JZ-BK) and recorded with a multichannel paper recorder (XWTD464). The rings were then washed and equilibrated for 30 min, and then preincubated for 30 min with 10^-5 mol/L of the specific NO biosynthesis inhibitor L-NNA. Contraction responses were evoked and measured with the range of cumulative concentrations of KCl and phenylephrine listed above in the presence of L-NNA. At the end of the experiments, the rings were weighed.

Quantification of NO₂^-

In principle, NO₂^- can react with 2, 3-diaminonathalen (DAN) in acid solution and produce a fluorescent substance, the intensity of which can be measured by a spectrofluorometer and used to indicate NONO₂^- content. To quantitate NO₂^-, we first made a standard curve by putting 100 μL aliquots of 0, 3.2 × 10^-7, 6.4 × 10^-7, 1.28 × 10^-6, 1.92 × 10^-6, 2.56 × 10^-6, 3.2 × 10^-6 and 3.84 × 10^-6-mol/L NaNO₂ into 5 mL test tubes, and then adding 10 μL of DAN-HCl solution, mixing well and allowing to react for 10 min at 20 °C. The reaction was stopped by adding 5 μL of 2.8 mol/L NaOH. Double-distilled water was then added to bring the final volume up to 3 mL, and the fluorescence intensity was measured at Ex365nm, Em420nm (RF-5000 Spectrofluorometer, Japan). Finally, the standard curve of fluorescence intensity versus the concentration of NaNO₂ was generated. To measurement the concentration of serum NO₂^-, 200 μL aliquots of serum were placed in test tubes and mixed with 200 μL chloroform. After standing for 5 min, each tube was centrifuged for 15 min at 3500 r/min. The resultant supernatant was then mixed with 20% of its volume of 20% sodium sulfosalicylate to remove proteins. After centrifuging for 15 min at 3500 r/min, 100 μL of the resultant supernatant was mixed with 50 μL of 0.01 mol/L EDTA and then 40 μL of DAN-HCl solution was added. After reacting at 20→ for 10 min, 5 μL of 2.8 mol/L NaOH was added to stop the reaction. Double-distilled water was added up to a final volume of 3 mL, and the fluorescence intensity was measured. The concentration of NO₂^- was calculated from the standard curve.

Statistical analysis

Dose-response contraction curves were made with the experimental data. The EC₅₀ values and the Rmax were calculated from each dose-response contraction curve by means of a non-linear regression method with a curve-fitting program. Rmax and EC₅₀ values were averaged and the results were assessed statistically using either the paired or unpaired Student’s t-test as appropriate. The data of NO₂^- were analyzed with Student’s t-test, and the results are presented as mean ± SEM. Statistical significance was set at a P level of 0.05 or less.

RESULTS

All the animals survived the modeling and sham operations and subsequent experimental course, except for one. No rats in the control or portal hypertensive model group showed any appreciable alteration in hepatic histology. Body weight, age, serum electrolytes, liver function and portal venous pressure are shown in Table 1. No differences were found between the partial portal vein constricted and control rats for the parameters of body weight, age, serum potassium, alanine aminotransferase (ALT) and albumin. However, compared with the controls, the portal hypertensive rats showed marked hyponatremia and elevated portal venous pressure (both P < 0.01).

Table 1 Baseline values in rats with portal hypertension and sham-operated control rats.

Parameter	Portal hypertension (n = 11)	Control (n = 8)
Body weight (g)	208 ± 17	201 ± 21
PVP (kPa)	1.644 ± 0.142b	1.034 ± 0.113
Serum Na+ (mmol/L)	135 ± 1b	141 ± 2
Serum K+ (mmol/L)	4.1 ± 0.2	4.3 ± 0.2
ALT (U/L)	147.6 ± 38.4	141.7 ± 53.3
Albumin (g/L)	16.43 ± 4.12	17.21 ± 3.84
A/G	0.64 ± 0.04	0.66 ± 0.08

^bP < 0.01 vs control.

Serum NO₂^- levels are shown in Table 2. Serum concentrations of NO₂^- in portal vein blood (0.766 ± 0.097 μmol/L) and peripheral blood (0.687 ± 0.092 μmol/L) were markedly elevated in the portal hypertensive rats, as compared with the levels in controls (0.613 ± 0.084 μmol/L and 0.591 ± 0.045 μmol/L respectively, P < 0.05). The level of NO₂^- in portal vein blood of portal hypertensive rats was significantly higher than that in peripheral blood (P < 0.05). The physical parameters of the aortic rings are shown in Table 3. The aortic rings of the portal hypertensive rats and the control rats showed no differences in external diameter, length or weight (P > 0.05).

Table 2 Serum levels of NO₂^- in rats with portal hypertension and sham-operated control rats (x ± s, ×10^-6 mol/L).

Group	n	Portal vein	Peripheral vein
PH	11	0.766 ± 0.097ac	0.687 ± 0.092a
Control	8	0.613 ± 0.084	0.591 ± 0.045

^aP < 0.05 vs control;

^cP < 0.05 vs peripheral vein blood.

Table 3 Physical characteristics of aortic rings from rats with portal hypertension and sham-operated control rats (x ± s).

Parameter	Portal hypertension	Control
ED (mm)	1.51 ± 0.04	1.49 ± 0.03
Length (mm)	3.09 ± 0.04	3.05 ± 0.07
Weight (mg)	1.59 ± 0.08	1.54 ± 0.08

ED: External diameter.

Contractile responses of abdominal aortic rings to KCl

Contractile responses to KCl were attenuated in rings from the portal hypertensive rats, as compared with those from the control rats, as evidenced by the dose response curves being shifted to the right (Figure 1). The EC₅₀ values were markedly higher in the aortic rings from the portal hypertensive rats (26.5 ± 0.9 mmol/L) than in those of the control rats (22.3 ± 1.7 mmol/L, P < 0.01). Maximal contraction was significantly diminished in the aortic rings of the portal hypertensive rats (0.72 ± 0.05 g), as compared with those of the control rats (0.98 ± 0.05 g, P < 0.01). After preincubation of the rings with L-NNA (10^-5 mol/L), the KCl-induced contractile curves were shifted to the left (compared with those made in the absence of L-NNA) for both the portal hypertensive and control rings. The difference in EC₅₀ values of the aortic rings was no longer statistically significant between the portal hypertensive rats (20.18 ± 0.8 mmol/L) and the control rats (19.4 ± 1.2 mmol/L, P > 0.05). However, the maximal KCl-induced contractions were still lower in the aortic rings from the portal hypertensive rats (1.08 ± 0.18 g) than in those from the control rats (1.21 ± 0.11 g, P < 0.05) (Table 4).

Figure 1 Dose response contraction curves for KCl in the presence of 10^-5 mol/L L-NNA in control and portal hypertensive abdominal aortic rings.

Table 4 Rmax and EC₅₀ values for KCl and phenylephrine in aortic rings from rats with portal hypertension and sham-operated control rats with and without L-NNA treatment (x ± s_x-).

Agent	EC50		Maximal contractions (g)
	Untreated	L-NNA	Untreated	L-NNA
KCl (mmol/L)
PH	26.5 ± 0.9ba	20.2 ± 0.8	0.72 ± 0.05ba	1.08 ± 0.1b
Control	22.3 ± 1.7a	19.4 ± 1.2	0.98 ± 0.05a	1.21 ± 0.11
PE (× 10-8 mol/L)
PH	3.72 ± 0.4ba	2.24 ± 0.18	1.04 ± 0.08ba	1.43 ± 0.14a
Control	2.81 ± 0.2b	2.18 ± 0.14	1.34 ± 0.39a	1.72 ± 0.11

^bP < 0.01 vs control;

^aP < 0.05 vs L-NNA.

Contractile responses of abdominal aortic rings to phenylephrine

Phenylephrine induced contractile curves were shifted to the right for the aortic rings from the portal hypertensive rats (Figure 2). The EC₅₀ values were markedly higher for the aortic rings from the portal hypertensive rats (37.2 ± 4 nmol/L) than those from the control rats (28.1 ± 2 nmol/L, P < 0.01). Maximal contractions were markedly lower in the aortic rings from the portal hypertensive rats (1.04 ± 0.08 g) than in those from the controls (1.34 ± 0.39 g, P < 0.01). After the rings were preincubated with L-NNA (10^-5 mol/L), the phenylephrine-induced contractile curves were shifted to the left for both portal hypertensive and control rings (Figure 2). The difference in EC₅₀ values of the aortic rings was no longer statistically significant between the portal hypertensive rats (22.4 ± 1.8 nmol/L) and the control rats (21.8 ± 1.4 nmol/L, P > 0.05). The maximal contractions were still lower in the portal hypertensive rings (1.43 ± 0.14 g) than in the control rings (1.72 ± 0.11 g, P < 0.05) (Table 4).

Figure 2 Dose-response contraction curves for phenylephrine in the presence of 10^-5 mol/L L-NNA in control and portal hypertensive abdominal aortic rings.

DISCUSSION

NO is synthesized from L-arginine by the enzyme NO synthase (NOS). There are two different types of NOS; the constitutive from (cNOS) is dependent on calcium and calmodulin for its activity, while the inducible from (iNOS) has no calcium or calmodulin requirement. cNOS is continuously present in the internal surface of the endothelial cell membrane, but iNOS represents a newly synthesized enzyme that is expressed in response to specific stimuli of endotoxin and cytokines in serial cell types, including vascular endothelium, vascular smooth muscle cells, hepatocytes and macrophages[3].

To date, no ideal technique is available to detect the NO content in tissues. The metabolic products of NO₃^-/NO₂^- are used to assess the NO level indirectly. The Griees reaction has been widely applied to measure NO₂^-, and it is relatively simple but its sensitivity is low[4]. In the present study, a new fluorometric analysis assay was developed and used to detect serum content of NO₂^-. Because NO₂^- plus 2, 3-diaminonaphthalene make a kind of fluorescent substance of 1-(H)-naphthotriazole, and the detection of the fluorescence intensity can reflect NO₂^- levels, the sensitivity of detection was markedly improved. The results of this study showed that serum levels of NO₂^-, especially those in portal vein blood, were elevated in rats with partial portal vein constriction, as compared with those in sham-operated controls. These findings indicated that there was an increased production of NO in portal hypertensive rats.

NO is an important regulator of systemic and local hemo-dynamics in physiological conditions, and is involved in the physiology of vascular tone, blood flow, velocity and vascular resistance. The mechanisms underlying these processes involve NO diffusion into the vascular smooth muscle cell, where it activates soluble guanylate cyclase and generates a subsequent increase in cGMP to cause relaxation of the vascular smooth muscle by binding of free intracellular calcium. Patients and experimental animals with portal hypertension reportedly present with hyperdynamic circulatory dysfunction characterized by splanchnic and systemic arterial vasodilation[5]. The pathogenesis of arterial vasodilation correlated with NO overproduction in portal hypertension. These findings collectively suggest that NO is an important mechanism of the hyperkinetic circulation associated with prehepatic portal hypertension.

L-NNA is an L-arginine analog, and competitively inhibits the activity of both iNOS and cNOS, subsequently attenuating NO release[6]. Our study found reduced contractile responses to both KCl and phenylephrine in abdominal aortic rings from partial portal vein constricted rats as compared with those from the sham-operated controls. Addition of the NOS inhibitor L-NNA (10^-5 mol/L) significantly increased the contractility to KCl and phenylephrine in both the control and portal hypertensive rings, but the contractile responses increased proportionally more in the portal hypertensive aortic rings than in the control rings in the presence of L-NNA. However, the difference in EC₅₀ values between the portal hypertensive and the control groups lost significance. These results suggest that vascular endothelium produces and releases some NO to maintain vasodilation under normal circumstances, while overproduction of NO in portal hypertensive vascular endothelium inhibits the vascular contractile response to KCl and phenylephrine. However, although the maximal KCl^- and phenylephrine-induced contractions were increased in rings from both portal hypertensive rats and control rats in the presence of L-NNA, the maximal contractions were still significantly lower in the portal hypertensive rings than those of the control rings. Thus, other factors, such as alteration in vascular smooth muscle, may play an important role in the pathogenesis of vasodilation and hyperdynamic circulation in portal hypertension.

The mechanism of increased vascular production of NO in rats with partial portal vein constriction cannot be shown from this study. The likely explanation is up-regulation of cNOS in the vascular endothelium, because iNOS is not expressed in isolated vessels which are not able to be stimulated by endotoxin and cytokines. In fact, increased cNOS activity has been reported by measuring NOS activity directly in homogenized blood vessels in animal models of portal hypertension with and without cirrhosis[7,8]. Whether the up-regulation of cNOS and subsequent NO overproduction is a primary or secondary event in the arteriolar vasodilation in portal hypertension could not be ascertained. Shear stress has been shown to be a powerful stimuli of cNOS[3]; therefore, overproduction of NO could be consequent to shear stress and other factors related to the hyperkinetic circulation. NO overproduction by endothelial cells would amplify and perpetuate arteriolar vasodilation. The effective inhibition of cNOS may reverse hyperdynamic circulation.

ACKNOWLEDGEMENTS

We are indebted to Dr. Ma Jin for his skillful technical assistance.